Obstet Gynecol Sci.  2016 Jul;59(4):307-310. 10.5468/ogs.2016.59.4.307.

Embryonic development after exposure of mouse oocyte to various amount of ovarian endometriotic fluid

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
  • 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. blasto@snubh.org
  • 3Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

This study assesses the fertilization and blastocyst-forming rate in mice cumulus-oocyte complexes (COCs) after the exposure of human ovarian endometriotic fluid. Endometriotic fluid was obtained from a single patient by aspiration at the time of a laparoscopic cystectomy and serially diluted. COCs were obtained from 46-week-old female BDF1 mice. After exposure to ovarian endometriotic fluid for five minutes, the COCs were washed three times and the oocytes were then fertilized by mice sperm. The fertilization and blastocyst formation rate and the proportion of hatching/hatched blastocyst in the four treatment groups were not inferior to those in non-exposure group.

Keyword

Blastocyst; Endometriotic fluid; Fertilization in vitro; Oocytes

MeSH Terms

Animals
Blastocyst
Cystectomy
Embryonic Development*
Female
Fertilization
Fertilization in Vitro
Humans
Mice*
Oocytes*
Pregnancy
Spermatozoa
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