J Dent Rehabil Appl Sci.  2016 Jun;32(2):117-122. 10.14368/jdras.2016.32.2.117.

365 nm LED laser treatment on beagle for gingival whitening without gum dermabrasion

• Lee SH
• Kim RK
• Seo NR
• Lim HK
• Byun SH
• Lim YJ
• Kim SM
• Lee JH

• KMID: 2328893
• DOI: http://doi.org/10.14368/jdras.2016.32.2.117

Abstract

PURPOSE
Gingival whitening is one of dental treatment purposes which is close to treating aesthetic disorders. Initial gingival whitening treatment was done by dermabrasion using a high power Diode Laser. However, this treatment method cannot be free from any infection or pain after the treatment. Therefore, we have decided to progress gingival whitening treatment using a low power LED laser.
MATERIALS AND METHODS
The laser was irradiated on pork meat then the safety of output power, temperature change and skin denaturalization was measured. Bison 365 nm LED laser was irradiated on oral mucosal pigment of a 15 - 20 kg beagle for 15 min for 1 - 2 weeks, one or two times each. Any pigment loss was checked through Hematoxyline-Eosin staining.
RESULTS
The melanin pigments at the area of 365 nm LED Laser irradiation were decreased.
CONCLUSION
The 365 nm LED Laser proposed in this study is considered to compensate the bleaching effect achieved by either using Diode laser or surgical methods.

Keyword

gingival whitening; melanocyte; laser treatment; 365 nm laser

MeSH Terms

Bison
Dermabrasion*
Gingiva*
Lasers, Semiconductor
Meat
Melanins
Melanocytes
Methods
Red Meat
Skin
Melanins

Figure

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  Fig. 1 Bison 365 nm LED laser output power & Temperature. (A, B) Change in output power when irradiated 365 nm laser on pork, (C, D) Measurement of change in temperature when irradiated 365 nm laser. (C) 3°C was not measured because it exceeded the 100 mW.

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  Fig. 2 Melanin pigment H/E staining. (A, D) 1 weeks and 2 weeks control groups. (B, E) It was melanin pigment that stained H/E in 1 weeks 1-time and 2 weeks 1-time irradiation. (C, F) It was melanin pigment that stained H/E in 1 weeks 2-time and 2 weeks 2-time irradiation.

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  Fig. 3 Melanin pigment counting statistical analysis. (A) Comparison of 1 weeks 1-time irradiation and 1 weeks 2-time (*, **P < 0.05). (B) Comparison of 2 weeks 1-time irradiation and 2 weeks 2-time (* P < 0.05). (C) Comparison of 1 weeks 1-time irradiation and 2 weeks 1-time irradiation (*, ** P < 0.05). (D) Comparison of 1 weeks 2-time irradiation and 2 weeks 2-time irradiation (* P < 0.01 and ** P < 0.05).

Reference

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