Nat Prod Sci.  2016 Jun;22(2):122-128. 10.20307/nps.2016.22.2.122.

Cytotoxic, Anti-Inflammatory and Adipogenic Effects of Inophyllum D, Calanone, Isocordato-oblongic acid, and Morelloflavone on Cell Lines

• Taher M
• Aminuddin
• Susanti D
• Aminudin N
• On S
• Ahmad F
• Hamidon H

• KMID: 2328878
• DOI: http://doi.org/10.20307/nps.2016.22.2.122

Abstract

This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordato-oblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavon e (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with ICâ‚…â‚€ of 84 µg/mL. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents.

Keyword

Cytotoxic; Anti-inflammatory; Adipogenic

MeSH Terms

Adenocarcinoma
Adipocytes
Breast
Calophyllum
Cell Line*
Coumarins
Garcinia
In Vitro Techniques
Macrophages
MCF-7 Cells
Coumarins

Figure

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  Fig. 1. Structure of tested compounds.

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  Fig. 2. Cytotoxic effects of selected flavonoids on MCF-7 cells by MTT assay. Results are presented as mean ± SD of triplicate of three experiments. (A) Calanone (B) Morelloflavone (C) Isocordato-oblongic acid (D) Inophyllum D.

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  Fig. 3. Cytotoxic effects of selected flavonoids on RAW 264.7 cells by MTT assay. Results are presented as mean ± SD of triplicate of three experiments.

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  Fig. 4. Anti-inflammatory effects of selected flavonoids on RAW 264.7 cells by nitrite determination test. The data shown were nitrite concentration against experimental groups for (A) Calanone (B) Morelloflavone (C) Isocordato-oblongic Acid (D) Inophyllum D. Results are presented as mean ± SD of triplicate from one independent experiment.

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  Fig. 5. Cytotoxic effects of selected flavonoids on 3T3 L1 pre-adipocyte. Results are presented as mean ± SD of triplicates of two repeated experiments.

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  Fig. 6. Effects of selected flavonoids on 3T3-L1 differentiation. All values were presented as mean ± SD indicating a measure of the dispersion of a set of data from its means from one independent experiment with three replications.

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  Fig. 7. Adipogenic effect of inophyllum D (A), calanone (B), morelloflavone (C) and isocordato-oblogic acid (D) at 25 µ g/mL on 3T3-L1 adipocyte. The cells were stained with Oil red O at 10 days after induction (400× magnification). Arrows indicated the presence of lipid droplet.

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