J Bacteriol Virol.  2016 Jun;46(2):99-103. 10.4167/jbv.2016.46.2.99.

Prevalence and Molecular Characteristics of Methicillin-resistant Staphylococcus aureus Isolates in a Neonatal Intensive Care Unit

Affiliations
  • 1Department of Clinical Laboratory Science, Catholic University of Pusan, Korea. smhwang@cup.ac.kr

Abstract

The molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolated from neonates in a neonatal intensive care unit (NICU) were investigated by multilocus sequence typing (MLST), staphylocoagulase (SC) genotyping, staphylococcal cassette chromosome mec (SCCmec) typing, accessory gene regulator (agr) typing, and the presence of Panton-Valentine leukocidin (PVL). Among the 44 S. aureus isolates from nares in neonates between March and June 2014 at hospital in Busan, 27 (61.4%) were MRSA and 17 (38.6%) were methicillin-susceptible S. aureus (MSSA). The most prevalent clone in MRSA isolates was ST72-SC type Vb-SCCmec IV-agr I (n=26) and the remaining one was ST89-SC type I-SCCmec II-agr II. In MSSA isolates, the prevalent clone was ST121-SC type Va-agr IV (n=13), followed by ST72-SC type Vb-agr I (n=2), ST8-SC type III-agr I (n=1) and ST15-SC type X-agr II (n=1). All isolates did not possess the PVL. The data showed that the neonates in NICU carried high prevalence of ST72 MRSA and remarkably different clones with SC diversity between MRSA and MSSA isolates.

Keyword

MRSA; NICU; Multilocus sequence type; Staphylocoagulase type; SCCmec type

MeSH Terms

Busan
Clone Cells
Coagulase
Humans
Infant, Newborn
Intensive Care, Neonatal*
Leukocidins
Methicillin Resistance*
Methicillin-Resistant Staphylococcus aureus*
Multilocus Sequence Typing
Prevalence*
Coagulase
Leukocidins

Reference

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