J Korean Acad Rehabil Med.  2008 Aug;32(4):375-387.

The Effect of Continuous Epidural Electrical Stimulation on Synapse and Neuronal Cell in Rat with Focal Ischemia

Affiliations
  • 1Department of Rehabilitation Medicine, Wonkwang University College of Medicine, Korea. cbrmshin@wonkwang.ac.kr
  • 2Department of Neurosurgery, Wonkwang University College of Medicine, Korea.
  • 3Department of Neurosurgery, Hallym University College of Medicine, Korea.
  • 4Department of Pathology, Wonkwang University College of Medicine, Institute of Wonkwang Medical Science, Korea.
  • 5Department of Anatomy, Chonbuk National University Medical School, Korea.

Abstract


OBJECTIVE
To evaluate the effects of continuous epidural electrical stimulation (ES) on the behavioral recovery, and the molecular proliferation of synapse and neural cell in rats with photothrombotic stroke. METHOD: The male Sprague-Dawley rats were pre-trained on a single pellet reaching task (SPRT), and then received the photothrombotic infarction on dominant sensorimotor cortex (SMC) and implantation of electrode over the peri-lesion SMC surface. All rats were randomly assigned to one of two groups: anodal ES on infarcted SMC (ES group) and no ES on infarcted SMC (control group). Rats received daily SPRT and neurological examinations for 14 days. After the rats had been sacrificed, brain sections were immunostained for quantification of infarct volumes and evaluation of the structural remodeling markers (MAP2, synaptophysin and GFAP).
RESULTS
The functional improvement of SPRT was significantly increased in the ES group compared to control group. There were no significant group differences in the infarct volumes, neurological examinations, structural remodeling markers. But, in the ES group, MAP2 and synaptophysin in affected peri-infarct area tended to increase compared with unaffected hemisphere. In affected hemisphere of ES group, many structural remodeling markers tended to increase compared with unaffected hemisphere. Especially, the staining of synaptophysin and GFAP in peri-infarct area showed more increased uptake than unaffected hemisphere in ES group and control group, respectively (p<0.05).
CONCLUSION
The ES improved greatly the behavioral motor function after SMC infarction and induced the significant synaptogenesis with the widespread neuronal proliferation in peri-infarct area. Postischemic astrogliosis was not remarkable in ES group.

Keyword

Cerebral Infarction; Stroke; Epidural electrical stimulation; Neural cell; Synapse
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