J Korean Soc Transplant.  2015 Jun;29(2):54-60. 10.4285/jkstn.2015.29.2.54.

Luminex-based Immunoassay for Organ Transplantation

• Lee H
• Oh EJ

• KMID: 2324352
• DOI: http://doi.org/10.4285/jkstn.2015.29.2.54

Abstract

Development of luminex-based solid phase assays enables advanced measurement of HLA antibody with sensitivity, specificity, and increasing knowledge of unacceptable antigens. In this review, we described the principle of the luminex-based assay and its current applications for organ transplantation including C1q assay, calculated panel reactive antibody, and virtual cross-matching. We also discussed the technical aspects and limitations for clinical utilization. The variables related to measurement of HLA antibody specificities and their clinical relevance remain unclear, therefore the interpretation of results requires comprehensive knowledge and clinical information in critical cases.

Keyword

Transplantation; Luminex; HLA antibody; Multiplex bead immunoassay

MeSH Terms

Antibody Specificity
Immunoassay*
Organ Transplantation*
Sensitivity and Specificity
Transplantation
Transplants*