J Korean Soc Transplant.  2003 Dec;17(2):131-136.

Increased Intracellular Reactive Oxygen Species in Peripheral Blood Mononuclear Cells from Renal Transplant Recipients with Decreased Graft Function

Affiliations
  • 1The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea.
  • 2BK21 for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
  • 3Hyonam Kidney Laboratory, Soon Chun Hyang University, Seoul, Korea.
  • 4Department of Surgery, Yonsei University Wonju College of Medicine, Wonju, Korea. yukim@yumc.yonsei.ac.kr

Abstract

PURPOSE
Oxidative stress plays an important role in the development and progression of renal injury. However, the role of reactive oxygen species (ROS) in renal allograft dysfunction is not clear. The present study examined the level of intracellular ROS in healthy control (kidney donor, n=37), end-stage renal disease (ESRD) patients (n=36), transplant recipients with serum creatinine (Scr) less than 1.5 mg% (n=33), and recipients with Scr between 1.5 and 5.0 mg% (n=36) at least one year after renal transplantation.
METHODS
Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque gradient method. Dichlorofluorescein (DCF)-sensitive ROS was measured by flow cytometry and expressed as an arbitrary unit.
RESULTS
Basal ROS production in PBMC was significantly increased in ESRD patients compared to healthy control. Basal ROS production in both transplant patient groups was not significantly different from healthy control. Phorbol-12-myristate-13- acetate (PMA) and hydrogen peroxide significantly enhanced intracellular ROS in all 4 groups. PMA- and hydrogen peroxide-induced cellular ROS was significantly higher in renal recipients with Scr between 1.5 and 5.0 mg% than in both healthy control and patients with Scr below 1.5 mg%. In regression analysis all, PMA- and hydrogen peroxide- induced as well as basal intracellular ROS in PBMC was correlated with Scr.
CONCLUSION
Our results demonstrate that oxidative stress correlates with the declining of renal graft function.

Keyword

Reactive oxygen species; Peripheral blood mononuclear cells; Transplantation; Graft function

MeSH Terms

Allografts
Creatinine
Flow Cytometry
Humans
Hydrogen
Hydrogen Peroxide
Kidney Failure, Chronic
Kidney Transplantation
Oxidative Stress
Reactive Oxygen Species*
Tissue Donors
Transplantation*
Transplants*
Creatinine
Hydrogen
Hydrogen Peroxide
Reactive Oxygen Species
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