J Korean Soc Spine Surg.  2000 Mar;7(1):1-8.

The Production of Phospholipase A2 in Different Types of Cultured Human Intervertebral Disc Cells

• Kim DJ
• Wang JM

Abstract

STUDY DESIGN: Evaluation of phospholipase A2 production according to cell type of human intervertebral disc. SUMMARY OF LITERATURE REVIEW: It was reported that the phospholipase A2 activity in human lumbar disc herniation was more active than that in other tissues.
OBJECTIVES
The purpose of this study was to evaluate the differences between the cells of anulus fibrosus and nucleus pulposus when lactate was added to the culture medium.
MATERIALS AND METHODS
Cells from the anulus fibrosus and nucleus pulposus of a human intervertebral disc were prepared enzymatically. After the monolayer was set up, the cells were divided to three groups and lactate doses of a 0mM, 2mM or 5mM were added respectively. At two week after lactate addition the production of phospholipase A2 was measured by Northern blotting.
RESULTS
Cells of nucleus pulposus produced a small amount of phospholipase A2. Those of anulus fibrosus showed a high activity of phospholipase A2 production. The concentration of lactate did not influenced on the production of phospholipase A2.
CONCLUSION
The anulus fibrosus has an important role in the production of phospholipase A2 and is thought to be related with generation of discogenic pain.

Keyword

Intervertebral disc; Cell culture; Lactate; Phospholipase A2

MeSH Terms

Blotting, Northern
Cell Culture Techniques
Humans*
Intervertebral Disc*
Lactic Acid
Phospholipases A2*
Phospholipases*
Lactic Acid
Phospholipases
Phospholipases A2