Yeungnam Univ J Med.  1995 Dec;12(2):246-259. 10.12701/yujm.1995.12.2.246.

Effect of Baclofen on the Cholinergic Nerve Stimulation in Isolated Rat Detrusor

• Lee KY
• Lee KM
• Choi EM
• Choi HC
• Ha JH
• Kim WJ

• KMID: 2320375
• DOI: http://doi.org/10.12701/yujm.1995.12.2.246

Abstract

This study aimed to investigate the mechanism of action of baclofen on the detrusor muscle isolated from rat. Rats (Sprague-Dawley) were sacrificed by decapitation and exsanguination. Horizontal muscle strips of 2 mm x 15mm were prepared for isometric myography in isolated muscle chamber bubbled with 95% / 5%-OZ / CO2 at 371C, and the pH was maintained at 7.4 Detrusor strips. contracted responding to the.. electrical field stimulation (EFS) by 2 Hz, 2U msec, monophasic square wave of 60 VDC. The initial peak of EFS-Induced contraction was tended to be suppresed by a,p-methylene-adenosine 5'-triphosphate (mATP), a partial agonist of purinergic receptor, and baclofen, a GABAB receptor agonist (statistically nonsignificant). The late sustained contraction by EFS was suppressed significantly (p < 0.05) by additions of atropione, a cholinergic muscarinic receptor antagonist and baclofen. The adenosine 5'-triphosphate-induced contraction was completely abolished by mA TP but not by baclofen. In the presence of atropine, the subsequent addition of acetylcholine could not contract the muscle strips: but the addition of acetylcholine in the presence of baclofen evoked a contraction to a remarkable extent.

MeSH Terms

Acetylcholine
Adenosine
Animals
Atropine
Baclofen*
Decapitation
Exsanguination
Hydrogen-Ion Concentration
Myography
Rats*
Receptors, Muscarinic
Acetylcholine
Adenosine
Atropine
Baclofen
Receptors, Muscarinic