Tuberc Respir Dis.  2012 Feb;72(2):132-139.

Overexpression of Periostin Protein in Non-Small Cell Lung Carcinoma is Not Related with Clinical Prognostic Significance

• Park WY
• Shin DH
• Kim JH
• Lee MK
• Lee HS
• Lee CH

Abstract

BACKGROUND
Periostin is preferentially expressed in periosteum, indicating a potential role in bone formation. Recently, there have been emerging controversies about its role in invasion and metastasis of human malignancies. We attempted to determine the clinicopathological significance of periostin expression in non-small cell lung carcinoma (NSCLC).
METHODS
Immunohistochemical staining of periostin protein from 91 cases of NSCLCs was performed using tissue microarray blocks. The results were correlated with clinicopathological parameters.
RESULTS
Positive reaction to periostin was predominantly noted in the tumor stroma. The strongest reaction presented as a band-like pattern just around the tumor nests. Non-neoplastic lung tissue and most in-situ carcinomas did not show a positive reaction in their stroma. With respect to tumor differentiation, moderate to poor differentiated tumors (47/77) revealed even higher periostin expression than the well-differentiated ones (4/14) (p=0.024). High periostin expression was positively correlated with E-cadherin and p53 expression, but was not related with patient age, sex, tumor type, PCNA index, b-catenin, cyclin D1, pTNM-T, pTNM-N, stage, and patient survival (p>0.05).
CONCLUSION
These results suggest that periostin might play a role during the biological progression of NSCLC, but may not be related to the clinical prognostic parameters.

Keyword

Carcinoma, Non-Small-Cell Lung; Cadherins; Genes, p53

MeSH Terms

Cadherins
Carcinoma, Non-Small-Cell Lung
Cyclin D1
Genes, p53
Humans
Lung
Neoplasm Metastasis
Osteogenesis
Periosteum
Proliferating Cell Nuclear Antigen
Cadherins
Cyclin D1
Proliferating Cell Nuclear Antigen

Figure

• 

  Figure 1 Periostin expression in tumors and intratumoral stroma (immunostain, ×200). (A) Non-neoplastic lung stroma shows negative (right) immunostaining in contrast to neoplastic stroma (left). (B) Intratumoral stoma around invasive adenocarcinoma shows positive immunoreaction. (C) The stroma of bronchioloalveolar carcinoma is negative for periostin. (D) Occasionally, the cytoplasm of adenocarcinoma cells shows positive reaction to periostin. (E) The strongest reaction in the positive stroma of squamous cell carcinoma shows a band-like pattern. (F) Intratumoral stroma of well to moderately differentiated squamous cell carcinoma shows negative staining to periostin.

• 

  Figure 2 Overall survival curves for patients with non-small cell lung carcinoma showing low expression (n=40) and high expression (n=51) of periostin.

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