Tuberc Respir Dis.  2011 Sep;71(3):195-201.

Comparison of Serum Osteopontin Levels in Patients with Stable and Chronic Obstructive Pulmonary Disease and Exacerbation

• Ma JE
• Lee SH
• Kim YE
• Lim SJ
• Lee SJ
• Jeong YY
• Kim HC
• Lee JD
• Hwang YS
• Cho YJ

Abstract

BACKGROUND
Osteopontin (Opn) is recognized as an important adhesive bone matrix protein and a key cytokine involved in immune cell recruitment and tissue repair and remolding. However, serum levels of osteopontin have not been evaluated in patients with chronic obstructive pulmonary disease (COPD). Thus, the aim of this study was to evaluate and compare the serum levels of osteopontin in patients experiencing COPD exacerbations and in patients with stable COPD.
METHODS
Serum samples were obtained from 22 healthy control subjects, 18 stable COPD patients, and 15 COPD with exacerbation patients. Serum concentrations of osteopontin were measured by the ELISA method.
RESULTS
Serum levels of osteopontin were higher in patients with acute exacerbation than with stable COPD and in healthy control subjects (62.4+/-51.9 ng/mL, 36.9+/-11.1 ng/mL, 30+/-11 ng/mL, test for trend p=0.003). In the patients with COPD exacerbation, the osteopontin levels when the patient was discharged from the hospital tended to decrease compared to those at admission (45+/-52.1 ng/mL, 62.4+/-51.9 ng/mL, p=0.160). Osteopontin levels significantly increased according to patient factors, including never-smoker, ex-smoker and current smoker (23+/-5.7 ng/mL, 35.5+/-17.6 ng/mL, 58.6+/-47.8 ng/mL, test for trend p=0.006). Also, osteopontin levels showed a significantly negative correlation with forced expiratory volume in one second (FEV1%) predicted in healthy controls and stable COPD patients (r=-0.389; p=0.013). C-reactive protein (CRP) was positively correlated with osteopontin levels in patients with COPD exacerbation (r=0.775; p=0.002).
CONCLUSION
The serum levels of osteopontin increased in patients with COPD exacerbation and tended to decrease after clinical improvement. These results suggest the possible role of osteopontin as a biomarker of acute exacerbation of COPD.

Keyword

Osteopontin; Pulmonary Disease, Chronic Obstructive; Disease Exacerbation; Biological Markers

MeSH Terms

Adhesives
Biomarkers
Bone Matrix
C-Reactive Protein
Disease Progression
Enzyme-Linked Immunosorbent Assay
Forced Expiratory Volume
Humans
Osteopontin
Pulmonary Disease, Chronic Obstructive
Adhesives
C-Reactive Protein
Osteopontin

Figure

• 

  Figure 1 Comparison of the OPN levels between healthy controls, stable COPD and COPD exacerbation. OPN level of patients with COPD exacerbation was much higher than those of healthy controls and stable COPD patients (30±11 ng/mL; 36.9±11.1 ng/mL; 62.4±51.9 ng/mL; test for trend, p=0.003). COPD: chronic obstructive pulmonary disease; OPN: Osteopontin.

• 

  Figure 2 Comparison of the OPN levels according to COPD severity. OPN level of stable COPD patients were increased according to COPD severity (30±11 ng/mL, 34.4±11.2 ng/mL; 43.3±8.4 ng/mL; 62.4±51.9 ng/mL; test for trend, p=0.002). OPN: osteopontin; COPD: chronic obstructive pulmonary disease. *p>0.05.

• 

  Figure 3 OPN levels were significantly higher, according to patients' status as never-smoked, ex-smoker, or current smoker (23±5.7 ng/mL; 35.5±17.6 ng/mL; 58.6±47.8 ng/mL; test for trend, p=0.006). OPN: osteopontin.

• 

  Figure 4 Relationship between CRP and OPN level in patients with COPD exacerbation. CRP was positively correlated with OPN level in patients with COPD exacerbation (r=0.775; p=0.002). CRP: C-reactive protein; COPD: chronic obstructive pulmonary disease; OPN: Osteopontin.

• 

  Figure 5 Relationship between PaO2 and OPN level in patients with COPD exacerbation. PaO2 was negatively correlated with OPN level in patients with COPD exacerbation (r=-0.775; p=0.002). OPN: osteopontin; COPD: chronic obstructive pulmonary disease.

• 

  Figure 6 Relationship between OPN and FEV1/FVC and FEV1. FEV1 percent predicted was significantly correlated with OPN level in healthy controls and stable COPD patients (r=-0.389; p=0.013). OPN: osteopontin; FEV1: forced expiratory volume in one second; FVC: force vital capacity; COPD: chronic obstructive pulmonary disease.

• 

  Figure 7 Changes of the OPN in patients with COPD exacerbation. OPN levels at the time of discharge from the hospital were tended to decrease when compared with those at the time of admission (45±52.1 ng/mL; 62.4±51.9 ng/mL; p=0.160). OPN: osteopontin; COPD: chronic obstructive pulmonary disease.

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