Saf Health Work.  2011 Mar;2(1):17-25.

Evaluation of the Genetic Toxicity of Cyclopentane and Ammonium Nitrate: In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells

Affiliations
  • 1Center for Chemical Safety and Health, Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency, Daejeon, Korea. rim3249@gmail.com

Abstract

OBJECTIVES: In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test.
METHODS
The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix).
RESULTS
Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method.
CONCLUSION
From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.

Keyword

Cyclopentane; Ammonium nitrate; Chromosomal aberration; In vitro; Chinese Hamster Ovary
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