Restor Dent Endod.  2013 Nov;38(4):210-214.

Chlorhexidine gel associated with papain in pulp tissue dissolution

  • 1Southwest Bahia State University-UESB, Jequie, Bahia, Brazil.
  • 2Master Endodontics, State University of Campinas-Unicamp, Campinas, Sao Paulo, Brazil.
  • 3Department of Health Southwest Bahia State University-UESB, Jequie, Bahia, Brazil.


This study aimed to evaluate the capacity of 2% chlorhexidine gel associated with 8% papain gel in comparison with 5.25% sodium hypochlorite in bovine pulp tissue dissolution.
Ninety bovine pulps of standardized sizes were used and fragmented into 5-mm sizes. The fragments were removed from the root middle third region. They were divided into 6 experimental groups (n = 15), 1) 8% papain; 2) 2% chlorhexidine; 3) 2% chlorhexidine associated with 8% papain; 4) 0.9% saline solution; 5) 2.5% sodium hypochlorite; and 6) 5.25% sodium hypochlorite. The pulp fragments were weighed and put into immobile test tubes for dissolution for time intervals of 30, 60, 90, and 120 min.
The 5.25% sodium hypochlorite had greater dissolution potential than the pure papain, and when associated with chlorhexidine, both promoted greater dissolution than did the saline solution and 2% chlorhexidine groups (p < 0.05). The 2.5% sodium hypochlorite promoted dissolution to a lesser extent than the groups with papain within a period of 30 min (p < 0.05), but, was comparable to the saline solution and chlorhexidine. After 120 min, the 2.5% and 5.25% sodium hypochlorite promoted dissolution of 100% of the pulp fragments, and papain, 61%, while chlorhexidine associated with papain and chlorhexidine alone dissolved only 55% and 3%, respectively.
The 8% papain in gel, both alone and in association with chlorhexidine, was able to dissolve bovine pulp tissue, but to a lesser extent than did 5.25% sodium hypochlorite.


Chlorhexidine; Endodontics; Papain

MeSH Terms

Sodium Chloride
Sodium Hypochlorite
Sodium Chloride
Sodium Hypochlorite


  • Figure 1 Percentage of pulp tissue dissolution in different time intervals.


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