Pediatr Allergy Respir Dis.  2007 Mar;17(1):8-16.

Effects of Mycoplasma Pneumoniae on Activation of Human Eosinophilic Leukaemia EoL-1 Cells

Affiliations
  • 1Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. kekim@yumc.yonsei.ac.kr

Abstract

PURPOSE: Mycoplasma pneumoniae is a common cause of lower respiratory disease, especially in children and young adults. Several studies have suggested that respiratory infection by M. pneumoniae is associated with reactive airway disease and asthma. Though eosinophilia in peripheral blood are revealed in patients with mycoplasmal pneumonia, what is not known is the functional capacity of M. pneumoniae to activate human eosinophils. We investigated whether M. pneumoniae lysate (MPL) can activate human eosinophils to release inflammatory mediators.
METHODS
Human eosinophilic leukemic cell lines, EoL-1 cells were incubated with MPL. Activation of EoL-1 cells was monitored by IL-8 production, superoxide production and surface expression of CD69, ICAM-1, CD11b, and CD49d. In addition, we examined the effect of MPL and the role of mitogen-activated protein kinases (MAPKs) on IL-8 expression in EoL- 1 cells.
RESULTS
MPL induced IL-8 release in a time- and dose- dependent manner. However MPL did not induce superoxide anion production and CD69, ICAM-1, CD11b, and CD49d surface expression in EoL-1 cells. Pretreatment with mitogen-activated protein/extracellular signal- regulated kinase (ERK) [MEK] inhibitor PD98059, c-Jun N-terminal kinase (JNK) inhibitor II SP600125, and selective p38 MAPK inhibitor SB202190 inhibited MPL-induced IL-8 production, but the MPL stimulation had no effect on the activities of nuclear factor (NF)-kappaB.
CONCLUSION
These observations suggest that MPL causes activation of EoL-1 cells, and activation of MAPKs by MPL may be one of the mechanisms that result in an increase of the production of IL-8.

Keyword

Mycoplasma pneumoniae; EoL-1 cells; Interleukin-8; Mitogen-activated protein kinases

MeSH Terms

Asthma
Cell Line
Child
Eosinophilia
Eosinophils*
Humans*
Intercellular Adhesion Molecule-1
Interleukin-8
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Mycoplasma pneumoniae*
Mycoplasma*
p38 Mitogen-Activated Protein Kinases
Phosphotransferases
Pneumonia
Pneumonia, Mycoplasma*
Superoxides
Young Adult
Intercellular Adhesion Molecule-1
Interleukin-8
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Phosphotransferases
Superoxides
p38 Mitogen-Activated Protein Kinases
Full Text Links
  • PARD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr