Pediatr Allergy Respir Dis.
1999 Sep;9(3):252-258.
Immunoblot Analysis of Allergens in Mosquito, Culex pipiens
- Affiliations
-
- 1Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
- 2Department of Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Pediatrics, Ajou University College of Medicine, Suwon, Korea.
Abstract
- PURPOSE
Adverse reactions to mosquito bites have been recognized for some time, and these usually consist of large swellings, generalized urticaria, angioedema, and less frequently asthma. There have been some efforts to define immunologic characteristics of mosquito allergens throughout the world. But, in Korea, the immunologic analysis of mosquito allergen have not been made.
METHODS
Extracts of locally distributed female mosquito Culex pipiens, were prepared from their heads and thoraxes. By sodium dodecylsulfate-polyacrylamide gel electrophoresis, proteins of two extracts were isolated. Then their proein bands were transferred to nitrocellulose membrane. Immunoblotting was performed with sera of allergic patients to mosquito bites, then completed by antibodies to human IgE.
RESULTS
Immunoblotting showed IgE binding proteins weighing 70 kD, 60 kD, 34 kD in Culex pipiens.
CONCLUSION
We found that mosquito antigens induced IgE response, and it suggested that species-specific antigens exist. Further investigation using salivary gland extracts from Culex genus are needed in identifying specific allergens.
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