Pediatr Allergy Respir Dis.  1999 Mar;9(1):93-99.

Normal Values of IgG and IgG Subclass Concentrations According to Age in Children

Affiliations
  • 1Department of Pediatrics, College of Medicine, Yonsei University, Korea.
  • 2Department of Clinical Pathology, College of Medicine, Yonsei University, Korea.
  • 3Green Medical, Korea.

Abstract

PURPOSE
Currently the most widely used method of measuring IgG concentration is the method employing ELISA. This method has an advantage to detect smaller quantities than other standard methods, but in certain cases, consistent results cannot be obtained, thus impairing reliable data analysis. In this study, we attempt to determine the advantages in data analysis offered by the new method developed by Binding Site Ltd. (England) that employs a nephelometry.
METHODS
20 healthy subjects were studied from each of the following age groups : neonates, 1-4 months old, 5-10 months old, 11-24 months old, and 2-5 years old children. Serum IgG and IgG subclass concentrations were measured by nephelometry (Gehring Nephelometer Analyzer II, Germany) using Human IgG subclass, Liquid reagents BNII kit (Binding Site Ltd., England).
RESULTS
1) The r values for the standard curves of IgG, IgG1, IgG2, IgG3, IgG4 concentrations were 0.991, 0.997, 0.980, 0.973, 0.997, respectively. 2) IgG, IgG3, and IgG4 concentrations were lowest at the age of 5-10 months and increased to normal adult levels at 2-5 years of age. 3) IgG1 and IgG2 were lowest at the age of 1-4 months and increased to normal adult levels at 2-5 years of age.
CONCLUSION
The method employing nephelometry for measuring serum IgG & IgG subclasses concentration is not as sensitive as ELISA in detecting the lower concentrations. However, our studies indicate that it presents the advantage of better quality control in measuring values in the average range.

Keyword

IgG; IgG subclasses; Nephelometry; Children

MeSH Terms

Adult
Binding Sites
Child*
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G*
Indicators and Reagents
Infant, Newborn
Nephelometry and Turbidimetry
Quality Control
Reference Values*
Statistics as Topic
Immunoglobulin G
Indicators and Reagents
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