Obstet Gynecol Sci.  2014 Nov;57(6):464-470. 10.5468/ogs.2014.57.6.464.

Protective effects of green tea polyphenol against cisplatin-induced nephrotoxicity in rats

• Ahn TG
• Kim HK
• Park SW
• Kim SA
• Lee BR
• Han SJ

• KMID: 2314012
• DOI: http://doi.org/10.5468/ogs.2014.57.6.464

Abstract

OBJECTIVE
This study is to compare the effects of green tea polyphenol (GTP) pre-treatment with those of GTP post-treatment on cisplatin (CP)-induced nephrotoxicity in rat.
METHODS
Male Sprague-Dawley rats were randomly divided into six groups. Animals in the control group received 0.9% saline (intraperitoneal); animals in the GTP group received 0.9% saline and GTP (0.2% GTP as their sole source of drinking water); the CP group received only CP (7 mg/kg, intraperitoneal); the CP+preGTP group received GTP from two days before CP to four days after CP and the CP+postGTP group received GTP for four days after CP. CP-induced renal toxicity was evaluated by plasma creatinine and blood urea nitrogen (BUN) concentrations; kidney tissue gamma-glutamyl transpeptidase (GGT) and alkaline phosphatase (AP) activities and histopathological examinations.
RESULTS
High serume creatinine and BUN concentrations were observed in CP treated rats. The GGT and AP activites were lower in kidney of CP treated rats compared to control rats. In addition, treatment with CP resulted in development of a marked tubular necrosis, and tubular dilation in kidney of rats. Pretreatment with GTP resulted in markedly reduced elevation of serum creatinine and BUN amounts and changes of GGT and AP activity in kidney induced by CP. CP-induced histopathological changes, including tubular necrosis and dilation, were ameliorated in GTP pre-treated rats, compared to CP alone or GTP post-treated rats.
CONCLUSION
These results demonstrate that GTP might have some protective effect against CP-induced nephrotoxicity in rat, and GTP pre-treatment was more effective than GTP post-treatment on reduction of CP-induced renal dysfunction.

Keyword

Cisplatin; Green tea polyphenol; Nephrotoxicity

MeSH Terms

Alkaline Phosphatase
Animals
Blood Urea Nitrogen
Cisplatin
Creatinine
Drinking
gamma-Glutamyltransferase
Guanosine Triphosphate
Humans
Kidney
Male
Necrosis
Plasma
Rats*
Rats, Sprague-Dawley
Tea*
Alkaline Phosphatase
Cisplatin
Creatinine
Guanosine Triphosphate
Tea
gamma-Glutamyltransferase

Figure

• 

  Fig. 1 The effects of dietary green tea polyphenol (GTP) on creatinine levels in cisplatin (CP) treated rats. Rats were administered normal saline (control, intraperitoneal injection), GTP (0.2% GTP drinking water) alone, CP alone (7 mg/kg body weight, intraperitoneal injection), CP+preGTP (GTP received from 2 days before CP to 4 days after CP) and CP+postGTP (GTP received 4 days after CP); creatinine levels were determined 4 days after these treatments. Values are mean±standard deviation (n=5). a,b,cValues with different superscripts are significantly different at the P<0.01 by Duncan's multiple range test.

• 

  Fig. 2 The effects of dietary green tea polyphenol (GTP) on blood urea nitrogen (BUN) amounts in cisplatin (CP) treated rats. Rats were administered normal saline (control, intraperitoneal injection), GTP (0.2% GTP drinking water) alone, CP alone (7 mg/kg body weight, intraperitoneal injection), CP+preGTP (GTP received from 2 days before CP to 4 days after CP) and CP+postGTP (GTP recieved 4 days after CP); BUN amounts were determined 4 days after these treatments. Values are mean±standard deviation (n=5). a,b,cValues with different superscripts are significantly different at the P<0.01 by Duncan's multiple range test.

• 

  Fig. 3 Histopathological analysis in renal cortex in the groups of rats studied: (A) control, (B) green tea extract (GTP), (C) CP+GTP pre-treatment (CP+preGTP), (D) cisplatin (CP), and (E) CP+GTP post-treatment (CP+postGTP). Rats were studied 4 days after CP injection (7 mg/kg). GTP was given in the drinking water (2 g/L) 2 days before and/or 4 days after CP injection (haematoxylin and eosin staining, ×200).

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