Nutr Res Pract.  2015 Jun;9(3):262-267. 10.4162/nrp.2015.9.3.262.

Hypoglycemic and antioxidant effects of Daraesoon (Actinidia arguta shoot) in animal models of diabetes mellitus

Affiliations
  • 1Department of Smart Food and Drugs, School of Food and Life Science, Inje University, 197 Inje-ro, Gimhae, 621-749, Korea. fdsnkiji@inje.ac.kr
  • 2Food and Nutrition Research Team, Division of Research and Development, Hurom Co., Ltd., 158 Golden root-ro, Gimhae, Gyungnam, 621-844, Korea.
  • 3Department of Food and Nutrition, Inha University, 100 Inha-ro, Incheon, 402-823, Korea.

Abstract

BACKGROUND/OBJECTIVES
The primary objective of the treatment of diabetes mellitus is the attainment of glycemic control. Hyperglycemia increases oxidative stress which contributes to the progression of diabetic complications. Thus, the purpose of this study was to investigate the hypoglycemic and antioxidant effects of Daraesoon (Actinidia arguta shoot) in animal models of diabetes mellitus.
MATERIALS/METHODS
Rats with streptozotocin-induced diabetes received an oral administration of a starch solution (1 g/kg) either with or without a 70% ethanol extract of Daraesoon (400 mg/kg) or acarbose (40 mg/kg) after an overnight fast and their postprandial blood glucose levels were measured. Five-week-old C57BL/6J mice were fed either a basal or high-fat/high-sucrose (HFHS) diet with or without Daraesoon extract (0.4%) or acarbose (0.04%) for 12 weeks after 1 week of adaptation to determine the effects of the chronic consumption of Daraesoon on fasting hyperglycemia and antioxidant status.
RESULTS
Compared to the control group, rats that received Daraesoon extract (400 mg/kg) or acarbose (40 mg/kg) exhibited a significant reduction in the area under the postprandial glucose response curve after the oral ingestion of starch. Additionally, the long-term consumption of Daraesoon extract or acarbose significantly decreased serum glucose and insulin levels as well as small intestinal maltase activity in HFHS-fed mice. Furthermore, the consumption of Daraesoon extract significantly reduced thiobarbituric acid reactive substances and increased glutathione levels in the livers of HFHS-fed mice compared to HFHS-fed mice that did not ingest Daraesoon.
CONCLUSIONS
Daraesoon effectively suppressed postprandial hyperglycemia via the inhibition of alpha-glucosidase in STZ-induced diabetic rats. Chronic consumption of Daraesoon alleviated fasting hyperglycemia and oxidative stress in mice fed a HFHS diet.

Keyword

Actinidia arguta shoot; alpha-glucosidase; glucose; antioxidant; diabetes

MeSH Terms

Acarbose
Administration, Oral
alpha-Glucosidases
Animals
Antioxidants*
Blood Glucose
Diabetes Complications
Diabetes Mellitus*
Diet
Eating
Ethanol
Fasting
Glucose
Glutathione
Hyperglycemia
Insulin
Liver
Mice
Models, Animal*
Oxidative Stress
Rats
Starch
Thiobarbituric Acid Reactive Substances
Acarbose
Antioxidants
Blood Glucose
Ethanol
Glucose
Glutathione
Insulin
Starch
Thiobarbituric Acid Reactive Substances
alpha-Glucosidases

Figure

  • Fig. 1 Incremental blood glucose levels (A) and area under the glucose response curve (B) of STZ-induced diabetic rats. Control group (•): Starch (1 g/kg) was administered orally to STZ-induced diabetic rats after an overnight fast. Daraesoon group (▴): Starch (1 g/kg) with 70% ethanol extract of Daraesoon (400 mg/kg) was administered orally to rats after an overnight fast. Acarbose group (▪): Starch (1 g/kg) plus acarbose (40 mg/kg) was administered orally to rats after an overnight fast. Values represent means ± SEM (n = 7). Means that do not share a common letter are significantly different at *P < 0.05 or **P < 0.01.

  • Fig. 2 Maltase activity in the small intestine in mice fed the experimental diets. Control, mice fed the basal diet; HFHS, mice fed a high-fat/high-sucrose (HFHS) diet; Daraesoon, mice fed the HFHS diet containing 0.4% Daraesoon extract; Acarbose, mice fed the HFHS diet containing 0.04% acarbose. Values represent means ± SEM (n = 7). Bars that do not share a common letter are significantly different at P < 0.05.


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