Nutr Res Pract.  2015 Apr;9(2):144-149. 10.4162/nrp.2015.9.2.144.

Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats

• Kim SY
• Kim H
• Min H

• KMID: 2313822
• DOI: http://doi.org/10.4162/nrp.2015.9.2.144

Abstract

BACKGROUND
/OBJECTIVE: The aim of this study was to examine the effect of high dietary methionine (Met) consumption on plasma and hepatic oxidative stress and dyslipidemia in chronic ethanol fed rats.
MATERIALS/METHODS
Male Wistar rats were fed control or ethanol-containing liquid diets supplemented without (E group) or with DL-Met at 0.6% (EM1 group) or 0.8% (EM2 group) for five weeks. Plasma aminothiols, lipids, malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase were measured. Hepatic folate, S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) were measured.
RESULTS
DL-Met supplementation was found to increase plasma levels of homocysteine (Hcy), triglyceride (TG), total cholesterol (TC), and MDA compared to rats fed ethanol alone and decrease plasma ALT. However, DL-Met supplementation did not significantly change plasma levels of HDL-cholesterol, cysteine, cysteinylglycine, and glutathione. In addition, DL-Met supplementation increased hepatic levels of folate, SAM, SAH, and SAM:SAH ratio. Our data showed that DL-Met supplementation can increase plasma oxidative stress and atherogenic effects by elevating plasma Hcy, TG, and TC in ethanol-fed rats.
CONCLUSION
The present results demonstrate that Met supplementation increases plasma oxidative stress and atherogenic effects by inducing dyslipidemia and hyperhomocysteinemia in ethanol-fed rats.

Keyword

Ethanol; DL-Methionine supplementation; oxidative stress; plasma lipids; aminothiols

MeSH Terms

Alanine Transaminase
Animals
Aspartate Aminotransferases
Cholesterol
Cysteine
Diet
Dyslipidemias*
Ethanol
Folic Acid
Glutathione
Homocysteine
Humans
Hyperhomocysteinemia
Male
Malondialdehyde
Methionine*
Oxidative Stress*
Plasma
Rats*
Rats, Wistar
S-Adenosylhomocysteine
S-Adenosylmethionine
Triglycerides
Alanine Transaminase
Aspartate Aminotransferases
Cholesterol
Cysteine
Ethanol
Folic Acid
Glutathione
Homocysteine
Malondialdehyde
Methionine
S-Adenosylhomocysteine
S-Adenosylmethionine