Nutr Res Pract.  2015 Feb;9(1):3-10. 10.4162/nrp.2015.9.1.3.

Intestinal anti-inflammatory activity of Sasa quelpaertensis leaf extract by suppressing lipopolysaccharide-stimulated inflammatory mediators in intestinal epithelial Caco-2 cells co-cultured with RAW 264.7 macrophage cells

• Kim KM
• Kim YS
• Lim JY
• Min SJ
• Ko HC
• Kim SJ
• Kim Y

• KMID: 2313802
• DOI: http://doi.org/10.4162/nrp.2015.9.1.3

Abstract

BACKGROUND/OBJECTIVES
Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, involves chronic inflammation of the gastrointestinal tract. Previously, Sasa quelpaertensis leaves have been shown to mediate anti-inflammation and anti-cancer effects, although it remains unclear whether Sasa leaves are able to attenuate inflammation-related intestinal diseases. Therefore, the aim of this study was to investigate the anti-inflammatory effects of Sasa quelpaertensis leaf extract (SQE) using an in vitro co-culture model of the intestinal epithelial environment.
MATERIALS/METHODS
An in vitro co-culture system was established that consisted of intestinal epithelial Caco-2 cells and RAW 264.7 macrophages. Treatment with lipopolysaccharide (LPS) was used to induce inflammation.
RESULTS
Treatment with SQE significantly suppressed the secretion of LPS-induced nitric oxide (NO), prostaglandin E2 (PGE2), IL-6, and IL-1beta in co-cultured RAW 264.7 macrophages. In addition, expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and tumor necrosis factor (TNF)-alpha were down-regulated in response to inhibition of IkappaBalpha phosphorylation by SQE. Compared with two bioactive compounds that have previously been identified in SQE, tricin and P-coumaric acid, SQE exhibited the most effective anti-inflammatory properties.
CONCLUSIONS
SQE exhibited intestinal anti-inflammatory activity by inhibiting various inflammatory mediators mediated through nuclear transcription factor kappa-B (NF-kB) activation. Thus, SQE has the potential to ameliorate inflammation-related diseases, including IBD, by limiting excessive production of pro-inflammatory mediators.

Keyword

Sasa quelpaertensis; co-culture model; anti-inflammation; pro-inflammatory mediators

MeSH Terms

Caco-2 Cells*
Coculture Techniques
Colitis, Ulcerative
Crohn Disease
Dinoprostone
Gastrointestinal Tract
Humans
Inflammation
Inflammatory Bowel Diseases
Interleukin-6
Intestinal Diseases
Macrophages*
Nitric Oxide
Nitric Oxide Synthase Type II
Phosphorylation
Prostaglandin-Endoperoxide Synthases
Sasa*
Transcription Factors
Tumor Necrosis Factor-alpha
Dinoprostone
Interleukin-6
Nitric Oxide
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases
Transcription Factors
Tumor Necrosis Factor-alpha