Nutr Res Pract.  2014 Oct;8(5):516-520. 10.4162/nrp.2014.8.5.516.

Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes

Affiliations
  • 1Department of Food & Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan, Chungnam 336-745, Korea.
  • 2Department of Obstetrics and Gynecology, Soonchunhyang University Cheonan Hospital, Soonchunhyang-6-gil 31, Dongnam-gu, Cheonan 330-721, Korea. sternum@schmc.ac.kr

Abstract

BACKGROUND/OBJECTIVES
Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes.
MATERIALS/METHODS
3T3-L1 adipocytes were treated with CA (0-20 microM) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured.
RESULTS
LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-alpha, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-kappaB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.
CONCLUSIONS
Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.

Keyword

3T3-L1 adipocytes; carnosic acid; inflammation; toll-like receptor 4

MeSH Terms

Adipocytes*
Adipokines
Chemokine CCL2
Inflammation
Interleukin-6
Myeloid Differentiation Factor 88
Obesity
Phosphotransferases
RNA, Messenger
TNF Receptor-Associated Factor 6
Toll-Like Receptor 4
Tumor Necrosis Factor-alpha
Up-Regulation
Adipokines
Chemokine CCL2
Interleukin-6
Myeloid Differentiation Factor 88
Phosphotransferases
RNA, Messenger
TNF Receptor-Associated Factor 6
Toll-Like Receptor 4
Tumor Necrosis Factor-alpha
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