Nutr Res Pract.  2014 Aug;8(4):404-409.

Effect of Hominis Placenta on cutaneous wound healing in normal and diabetic mice

• Park JY
• Lee J
• Jeong M
• Min S
• Kim SY
• Lee H
• Lim Y
• Park HJ

Abstract

BACKGROUND/OBJECTIVES
The number of diabetic patients has recently shown a rapid increase, and delayed wound healing is a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment was investigated in normal and streptozotocin-induced diabetic mice.
MATERIALS/METHODS
Four full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injected subcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound size was measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Wound tissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichrome staining for collagen study.
RESULTS
Significantly faster wound closure rates were observed in the HP treated group than in normal and diabetes control mice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6 in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significant increase in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treated groups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in both normal and diabetic mice.
CONCLUSIONS
Taken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulation of the entire wound healing process, including inflammation, proliferation, and remodeling.

Keyword

Diabetes; wound; Hominis placenta; inflammation; fibroblast growth factor 2

MeSH Terms

Acceleration
Animals
Biopsy
Collagen
Fibroblast Growth Factor 2
Fibroblast Growth Factors
Humans
Immunohistochemistry
Inflammation
Mice*
Placenta*
Skin
Wound Healing*
Wounds and Injuries
Collagen
Fibroblast Growth Factor 2
Fibroblast Growth Factors

Figure

• 

  Fig. 1 Effects of Hominis placenta treatment on wound closure in normal and diabetic mice. Values with different superscripts are significantly different by 2-way ANOVA followed by least significant difference test (*P < 0.05, ***P < 0.001). * means statistical difference vs. group W. Values are expressed as mean ± SD. (A) N/W: untreated group after wounding, N/W+P: Hominis placenta treated group after wounding. (B) DB/W: untreated diabetes group after wounding, DB/W+P: Hominis placenta treated diabetes group after wounding.

• 

  Fig. 2 Changes of inflammatory cells in normal and diabetic mice. Values with different superscripts are significantly different by 2-way ANOVA followed by least significant difference test (*P < 0.05, **P < 0.01). * means statistical difference vs. group W. Values are expressed as mean ± SD. (A, C) N/W: untreated group after wounding, N/W+P: Hominis placenta treated group after wounding. (B, D) DB/W: untreated diabetes group after wounding, DB/W+P: Hominis placenta treated diabetes group after wounding.

• 

  Fig. 3 Changes of FGF2 expression in normal and diabetic mice. Values with different superscripts are significantly different by 2-way ANOVA followed by least significant difference test (*P < 0.05, **P < 0.01). * means statistical difference vs. group W. Values are expressed as mean ± SD. (A, C) N/W: untreated group after wounding, N/W+P: Hominis placenta treated group after wounding. (B, D) DB/W: untreated diabetes group after wounding, DB/W+P: Hominis placenta treated diabetes group after wounding.

• 

  Fig. 4 Changes of collagen at the granulation tissue and dermis in normal and diabetic mice. (A) N/W: untreated group after wounding, N/W+P: Hominis placenta treated group after wounding. (B) DB/W: untreated diabetes group after wounding, DB/W+P: Hominis placenta treated diabetes group after wounding. Red: keratin and muscle fibers, blue: collagen and bone, light red: cytoplasm, black: cell nuclei.

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