Nutr Res Pract.  2013 Dec;7(6):446-452.

Chamnamul [Pimpinella brachycarpa (Kom.) Nakai] ameliorates hyperglycemia and improves antioxidant status in mice fed a high-fat, high-sucrose diet

• Lee SJ
• Choi HN
• Kang MJ
• Choe E
• Auh JH
• Kim JI

Abstract

Chronic consumption of a high-fat, high-sucrose (HFHS) diet increases insulin resistance and results in type 2 diabetes mellitus in C57BL/6J mice. Hyperglycemia in diabetics increases oxidative stress, which is associated with a high risk of diabetic complications. The purpose of this study was to examine the hypoglycemic and antioxidant effects of chamnamul [Pimpinella brachycarpa (Kom.) Nakai] in an animal model of type 2 diabetes. The alpha-glucosidase inhibitory activity of a 70% ethanol extract of chamnamul was measured in vitro. Five-week-old male C57BL/6J mice were fed a basal or HFHS diet with or without a 70% ethanol extract of chamnamul at a 0.5% level of the diet for 12 weeks after 1 week of adaptation. After sacrifice, serum glucose, insulin, adiponectin, and lipid profiles, and lipid peroxidation of the liver were determined. Homeostasis model assessment for insulin resistance (HOMA-IR) was determined. Chamnamul extract inhibited alpha-glucosidase by 26.7%, which was 78.3% the strength of inhibition by acarbose at a concentration of 0.5 mg/mL. Serum glucose, insulin, and cholesterol levels, as well as HOMA-IR values, were significantly lower in the chamnamul group than in the HFHS group. Chamnamul extract significantly decreased the level of thiobarbituric acid reactive substances and increased the activities of superoxide dismutase, catalase, and glutathione peroxidase in the liver compared with the HFHS group. These findings suggest that chamnamul may be useful in prevention of hyperglycemia and reduction of oxidative stress in mice fed a HFHS diet.

Keyword

Chamnamul [Pimpinella brachycarpa (Kom.) Nakai]; glucose; insulin; cholesterol; antioxidant effect

MeSH Terms

Acarbose
Adiponectin
alpha-Glucosidases
Animals
Antioxidants
Blood Glucose
Catalase
Cholesterol
Diabetes Complications
Diabetes Mellitus, Type 2
Diet*
Ethanol
Glucose
Glutathione Peroxidase
Homeostasis
Humans
Hyperglycemia*
Insulin
Insulin Resistance
Lipid Peroxidation
Liver
Male
Mice*
Models, Animal
Oxidative Stress
Superoxide Dismutase
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
Acarbose
Adiponectin
Antioxidants
Catalase
Cholesterol
Ethanol
Glucose
Glutathione Peroxidase
Insulin
Superoxide Dismutase
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
alpha-Glucosidases

Figure

• 

  Fig. 1 Inhibitory activities of chamnamul against yeast α-glucosidase. The inhibitory activities of the 70% ethanol extracts of chamnamul and acarbose were measured at a concentration of 0.5 mg/mL. Values represent means ± SEM of triplicate measurements.

• 

  Fig. 2 Lipid peroxide levels and activities of antioixdative enzymes of the liver in the mice fed the experimental diets. (A) TBARS, (B) SOD activity, (C) CAT activity and (D) GSH-Px activity. Groups are the same as in Table 2. Values are presented as mean ± SEM (n = 7). Significantly different at P < 0.05 (*) and P < 0.01 (**).

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