Nutr Res Pract.  2010 Apr;4(2):99-105.

Sasa borealis leaves extract improves insulin resistance by modulating inflammatory cytokine secretion in high fat diet-induced obese C57/BL6J mice

Affiliations
  • 1Department of Food and Nutrition, Chonnam National University, 300 Youngbong-dong, Buk-gu, Gwangju 500-757, Korea. yrhuh@jnu.ac.kr

Abstract

Obesity is considered a mild inflammatory state, and the secretion of inflammation-related cytokines rises as adipose tissue expands. Inflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), interlukin 6 (IL-6) and monocyte-chemoattractant protein 1 (MCP-1), are modulated by adipose tissue and known to play an important role in insulin resistance which is the common characteristics of obesity related disorders. In this study we analyzed the effects of Sasa borealis leaves extract on inflammatory cytokines and insulin resistance in diet induced obese C57/BL6J mice. The obese state was induced by a high fat diet for 20 weeks and then the mice were divided into two groups; obese control group (OBC, n = 7) and experimental group (OB-SBE, n = 7). The OBC group was fed a high fat diet and the OB-SBE group was fed a high fat diet containing 5% Sasa borealis leaves extract (SBE) for 12 weeks. We also used mice fed a standard diet as a normal control (NC, n = 7). The body weight and adipose tissue weight in the OB group were significantly higher than those in the NC group. The effects of the high fat diet were reduced by SBE treatments, and the body weight and adipose tissue deposition in the OB-SBE group were significantly decreased compared to the OBC group. The OBC group showed higher serum glucose and insulin levels which resulted in a significant increase of incremental area under the curve (IAUC) and HOMA-IR than the NC group. Also, serum leptin, TNF-alpha, and IL-6 levels were significantly higher in the OBC group than in the NC group. In contrast, the OB-SBE group showed a reversal in the metabolic defects, including a decrease in glucose, insulin, IAUC, HOMA-IR, TNF-alpha, IL-6 and leptin levels. These results suggest that BSE can suppress increased weight gain and/or fat deposition induced by a high fat diet and theses effects are accompanied by modulation of the inflammatory cytokines, TNF-alpha and IL-6 secretion resulting in improved insulin resistance.

Keyword

Obesity; insulin resistance; inflammation; cytokine; Sasa borealis

MeSH Terms

Adipose Tissue
Animals
Body Weight
Cytokines
Diet
Diet, High-Fat
Glucose
Inflammation
Insulin
Insulin Resistance
Interleukin-6
Leptin
Mice
Obesity
Sasa
Tumor Necrosis Factor-alpha
Weight Gain
Cytokines
Glucose
Insulin
Interleukin-6
Leptin
Tumor Necrosis Factor-alpha

Figure

  • Fig. 1 Changes of body weight during the experimental periods. Group abbreviation: NC; mice fed normal diet, OBC; mice fed the high fat diet, OB-SBE; mice fed the diet containing 5% Sasa borealis leaves extract. Mean values with * or different letters are significantly different by repeated-measures ANOVA at P < 0.05.

  • Fig. 2 The effects of SBE on incremental blood glucose levels (left) and incremental area under the curve (right) after oral glucose tolerance test. Group abbreviation: NC; mice fed normal diet, OBC; mice fed the high fat diet, OB-SBE; mice fed the diet containing 5% Sasa borealis leaves extract. Mean values with different letters are significantly different between the three groups by ANOVA with Tukey's test at P < 0.05.

  • Fig. 3 The effects of SBE on serum inflammatory cytokine levels. Group abbreviation: NC; mice fed normal diet, OBC; mice fed the high fat diet, OB-SBE; mice fed the diet containing 5% Sasa borealis leaves extract. Mean values with different letters are significantly different between the three groups by ANOVA with Tukey's test at P < 0.05.


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