Mycobiology.  2015 Dec;43(4):450-457. 10.5941/MYCO.2015.43.4.450.

Differential Modulation of Lipopolysaccharide-Induced Inflammatory Cytokine Production by and Antioxidant Activity of Fomentariol in RAW264.7 Cells

• Seo DW
• Yi YJ
• Lee MS
• Yun BS
• Lee SM

• KMID: 2312849
• DOI: http://doi.org/10.5941/MYCO.2015.43.4.450

Abstract

Medicinal mushrooms have been used worldwide to treat cancer and modulate the immune system. Over the last several years, there has been increasing interest in isolating bioactive compounds from medicinal mushrooms and evaluating their health beneficial effects. Fomes fomentarius is used in traditional oriental medicine and is known to possess antioxidant, anti-inflammatory, antidiabetic, and antitumor effects. In the present study, we isolated fomentariol from Fomes fomentarius and investigated its anti-inflammatory effect in murine macrophages (RAW264.7 cells) stimulated with lipopolysaccharides. Fomentariol inhibited the production of nitric oxide and intracellular reactive oxygen species triggered by lipopolysaccharides. Interestingly, fomentariol differentially regulated cytokine production triggered by lipopolysaccharides. Fomentariol effectively suppressed the production of interleukin-1beta and interleukin-6 but not tumor necrosis factor-alpha. The inhibitory effect of fomentariol against nitric oxide, interleukin-1beta, and interleukin-6 production was possibly mediated by downregulation of the extracellular signal-regulated kinase signaling pathway. Taken together, our results suggest that fomentariol differentially modulated inflammatory responses triggered by lipopolysaccharides in macrophages and is one of the bioactive compounds that mediate the physiological effects of Fomes fomentarius.

Keyword

ERK signaling pathway; Fomes fomentarius; Fomentariol; LPS-induced inflammatory responses; RAW264.7 cells

MeSH Terms

Agaricales
Coriolaceae
Down-Regulation
Immune System
Interleukin-1beta
Interleukin-6
Lipopolysaccharides
Macrophages
Medicine, East Asian Traditional
Nitric Oxide
Phosphotransferases
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Interleukin-1beta
Interleukin-6
Lipopolysaccharides
Nitric Oxide
Phosphotransferases
Reactive Oxygen Species
Tumor Necrosis Factor-alpha