Mycobiology.  2015 Dec;43(4):435-443. 10.5941/MYCO.2015.43.4.435.

Phylogenetic Diversity and Antifungal Activity of Endophytic Fungi Associated with Tephrosia purpurea

• Luo ZP
• Lin HY
• Ding WB
• He HL
• Li YZ

• KMID: 2312847
• DOI: http://doi.org/10.5941/MYCO.2015.43.4.435

Abstract

Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.

Keyword

Antifungal activity; Endophytic fungi; ITS sequence; Tephrosia purpurea

MeSH Terms

Ascomycota
Colon
Fungi*
Plants
Sequence Analysis
Tephrosia*