Mycobiology.  2011 Jun;39(2):118-120.

Detection of Extracellular Enzyme Activities in Ganoderma neo-japonicum

Affiliations
  • 1Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea. jws67@korea.kr
  • 2Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horicultural & Herbal Science, Rural Development Administration, Suwon 404-707, Korea.
  • 3College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.

Abstract

The ability of Ganoderma to produce extracellular enzymes, including beta-glucosidase, cellulase, avicelase, pectinase, xylanase, protease, amylase, and ligninase was tested in chromogenic media. beta-glucosidase showed the highest activity, among the eight tested enzymes. In particular, Ganoderma neo-japonicum showed significantly stronger activity for beta-glucosidase than that of the other enzymes. Two Ganoderma lucidum isolates showed moderate activity for avicelase; however, Ganoderma neo-japonicum showed the strongest activity. Moderate ligninase activity was only observed in Ganoderma neo-japonicum. In contrast, pectinase, amylase, protease, and cellulase were not present in Ganoderma. The results show that the degree of activity of the tested enzymes varied depending on the Ganoderma species tested.

Keyword

Chromogenic media; Extracellular enzymes; Ganoderma neo-japonicum

MeSH Terms

Amylases
beta-Glucosidase
Cellulase
Cellulases
Ganoderma
Oxygenases
Polygalacturonase
Reishi
Amylases
Cellulase
Cellulases
Oxygenases
Polygalacturonase
beta-Glucosidase
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