Mycobiology.
2011 Mar;39(1):64-66.
Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates
- Affiliations
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- 1Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Korea. htchoi@kangwon.ac.kr
Abstract
- Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.
MeSH Terms
-
Carbohydrate Dehydrogenases
DNA, Complementary
Fungi
Glucose
Glucose Oxidase
Humans
Hydrogen
Hydrogen Peroxide
Lignin
Manganese
Oxidoreductases
Peroxidase
Peroxidases
Polymerase Chain Reaction
Pyrrolidines
Carbohydrate Dehydrogenases
DNA, Complementary
Glucose
Glucose Oxidase
Hydrogen
Hydrogen Peroxide
Lignin
Manganese
Oxidoreductases
Peroxidase
Peroxidases
Pyrrolidines
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