Mycobiology.  2007 Mar;35(1):21-24.

Comparison of Dyes for Easy Detection of Extracellular Cellulases in Fungi

Affiliations
  • 1Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, Korea. piceae@naver.com
  • 2Korean Agricultural Culture Collection and National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.

Abstract

To evaluate which dye is effective in a plate assay for detecting extracellular cellulase activity produced by fungi, four chromogenic dyes including remazol brilliant blue, phenol red, congo red, and tryphan blue, were compared using chromagenic media. For the comparison, 19 fungal species belonging to three phyla, ascomycota, basidiomycota, and zygomycota were inoculated onto yeast nitrogen-based media containing different carbon substrates such as cellulose (carboxylmethyl and avicel types) and cellobiose labeled with each of the four dyes. Overall, the formation of clear zone on agar media resulting from the degradation of the substrates by the enzymes secreted from the test fungi was most apparent with media containing congo red. The detection frequency of cellulase activity was also most high on congo red-supplemented media. The results of this study showed that congo red is better dye than other three dyes in a plate assay for fungal enzyme detection.

Keyword

Cellulase; Chromogenic media; Congo red; beta-Glucosidase

MeSH Terms

Agar
Ascomycota
Basidiomycota
beta-Glucosidase
Carbon
Cellobiose
Cellulase
Cellulases*
Cellulose
Coloring Agents*
Congo
Congo Red
Fungi*
Phenolsulfonphthalein
Yeasts
Agar
Carbon
Cellobiose
Cellulase
Cellulases
Cellulose
Coloring Agents
Congo Red
Phenolsulfonphthalein
beta-Glucosidase
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