Mycobiology.  2006 Jun;34(2):45-55.

Comparison of RAPD, AFLP, and EF-1alpha Sequences for the Phylogenetic Analysis of Fusarium oxysporum and Its formae speciales in Korea

• Park JM
• Kim GY
• Lee SJ
• Kim MO
• Huh MK
• Lee TH
• Lee JD

Abstract

Although Fursarium oxysporum causes diseases in economically important plant hosts, identification of F. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. To resolve these complexity, we evaluated genetic relationship of nine formae speciales of F. oxysporum with random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and translation elongation factor-1 alpha (EF-1alpha) gene. In addition, the correlation between mycotoxin content of fusaric acid and isolates based on molecular marker data was evaluated using the modified Mantel's test. According to these result, these fusaric acid-producing strains could not identify clearly, and independent of geographic locations and host specificities. However, in the identification of F. oxysporum formae speciales, especially, AFLP analysis showed a higher discriminatory power than that of a the RAPD and EF-1alpha analyses, all three techniques were able to detect genetic variability among F. oxysporum formae speciales in this study.

Keyword

Amplified fragment length polymorphism; Fusarium oxysporum ; Genetic variability; Random amplified polymorphic DNA; Translation elongation factor-1 alpha

MeSH Terms

Amplified Fragment Length Polymorphism Analysis
Classification
DNA
Fusaric Acid
Fusarium*
Geographic Locations
Host Specificity
Korea*
Peptide Elongation Factor 1*
Plants
DNA
Fusaric Acid
Peptide Elongation Factor 1