Mycobiology.
2004 Mar;32(1):6-10.
Molecular Marker Related to Fruitbody Color of Flammulina velutipes
- Affiliations
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- 1Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA, Suwon 441-707, Korea. wskong@rda.go.kr
- 2Department of Biotechnology, Yonam College of Agriculture, Sunghwan-Eup, Chonan 330-802, Korea.
- 3Department of Agronomy, Konkuk University, Seoul 143-701, Korea.
Abstract
- White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.
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