Mycobiology.
2003 Dec;31(4):235-247.
DNA Fingerprinting Analysis of the Genus Phytophthora in Korea
- Affiliations
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- 1Bioinformatics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea. dspark@rda.go.kr
- 2Graduate School of Bio and Information Technology, Hankyoung National University, Ansung 456-749, Korea.
- 3Molecular Physiology Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
Abstract
- In order to investigate biodiversity and establish identification system for Phytophthora spp. in Korea, a variety of band pattern was produced by using the URP (universal rice primer). The fingerprint patterns of Phytophthora spp. showed many common and variable fragments according to their isolates in distinct genotypes. In particular, P. drechsleri was classified into four distinct types (I to IV). P. drechsleri (KACC 40498 and KACC 40499) and P. cryptogea (KACC 40413) appeared to have almost equal bands despite their being different species. Ninety isolates of Phytophthora spp. were clustered into 13 groups based on UPGMA (unweighted pair group method with arithmetic means) analysis. These DNA fingerprinting data would be helpful for inter- and intra-species identification of Phytophthora species.
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