Mycobiology.  2000 Dec;28(4):171-176.

Genomic Organization of ancop Gene for alpha-COP Homolog from Aspergillus nidulans

Abstract

We have cloned a alpha-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using alpha-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved region of the amino acid sequences of Saccharomyces cerevisiae alpha-COP, Homo sapiens HEP-COP, and Drosophila melanogaster alpha-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other alpha-COPs gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsis . In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.

Keyword

Aspergillus nidulans; alpha-COP; Genomic structure; Sequence homology

MeSH Terms

Amino Acid Sequence
Arabidopsis
Aspergillus nidulans*
Aspergillus*
Base Sequence
Clone Cells
Coatomer Protein*
DNA
DNA, Complementary
Drosophila
Drosophila melanogaster
Humans
Introns
Saccharomyces cerevisiae
Sequence Homology
Transcription Initiation Site
Coatomer Protein
DNA
DNA, Complementary
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