Lab Med Online.  2016 Jan;6(1):36-40. 10.3343/lmo.2016.6.1.36.

Evaluation of Automated Specimen Inoculation for Blood Culture Samples by Use of the Previ Isola(R) System Compared with the Manual Method

Affiliations
  • 1Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon, Korea. shkoo@cnu.ac.kr

Abstract

BACKGROUND
Recently, a new automated inoculating instrument, Previ Isola(R) (bioMerieux, France) was introduced. Although there are many evaluation reports about the inoculation of urine and body fluid samples using Previ Isola(R), no evaluation has been reported for blood samples. The objectives of this study were to evaluate this instrument for the inoculation of blood samples and to compare the microbiological results with the manual loop-to-plate method.
METHODS
From March 2014 to July 2014, a total of 296 non-duplicate blood samples showing positive signals on the BacT/Alert 3D system were obtained, and both manual and automated methods were used for sample inoculation. Results of the two methods were compared according to five aspects: the culture result, number of single colonies, morphology of colonies, number of re-inoculations, and time required for inoculation.
RESULTS
The sensitivity and specificity of Previ Isola(R) were 98.9% and 96.6%, respectively. The positive and negative predictive values were 99.6% and 90.3%, respectively, and the total concordance rate was 98.6%. For Previ Isola(R) and the manual methods, the number of average usable single colonies per plate was 25 and 16, the number of re-inoculations was 60 and 62, and the inoculation time for 15 blood samples was 30 min and 75 min, respectively. The morphology of colonies showed no differences between the two methods.
CONCLUSIONS
The automated inoculation instrument, Previ Isola(R), showed relative good concordance with manual method, with high sensitivity and high specificity for blood sample inoculation. Previ Isola(R) may be useful for inoculating specimens including blood samples.

Keyword

Previ Isola(R); Automation; Inoculation; Blood culture

MeSH Terms

Automation
Body Fluids
Evaluation Studies as Topic
Sensitivity and Specificity

Reference

1. Chong Y, Lee K, et al. Diagnostic microbiology. 4th ed. Seoul: Seoheung Publishing Company;2009. p. 58–113.
2. Glasson JH, Guthrie LH, Nielsen DJ, Bethell FA. Evaluation of an automated instrument for inoculating and spreading samples onto agar plates. J Clin Microbiol. 2008; 46:1281–1284.
Article
3. Greub G, Prod'hom G. Automation in clinical bacteriology: what system to choose. Clin Microbiol Infect. 2011; 17:655–660.
Article
4. Mischnik A, Mieth M, Busch CJ, Hofer S, Zimmermann S. First evaluation of automated specimen inoculation for wound swab samples by use of the Previ Isola system compared to manual inoculation in a routine laboratory: finding a cost-effective and accurate approach. J Clin Microbiol. 2012; 50:2732–2736.
Article
5. Uh Y, Jang IH, Park SD, Kim KS, Seo DM, Yoon KJ, et al. Factors influencing the false positive signals of continuous monitoring blood culture system. Ann Clin Microbiol. 2014; 17:58–64.
Article
6. Nebbad-Lechani B, Emirian A, Maillebuau F, Mahjoub N, Fihman V, Legrand P, et al. New procedure to reduce the time and cost of broncho-pulmonary specimen management using the Previ Isola® automated inoculation system. J Microbiol Methods. 2013; 95:384–388.
Article
7. Kim Y, Yoon S, Sohn YS, Lee Y, Chung HS, Lee W, et al. Evaluation of an automated instrument, PREVI Isola® for inoculation of body fluids and urine samples onto agar plates. Lab Med Online. 2011; 1:105–109.
Article
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