Lab Med Online.  2013 Oct;3(4):198-212.

Analytical Performance of Wako and Sekisui Clinical Chemistry Assays on Hitachi LABOSPECT 008

• Chae H
• Park HI
• Kim M
• Lee J
• Kim Y

Abstract

BACKGROUND
We evaluated the analytical performance of Wako assays for albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), complement C3 and C4, calcium, creatine kinase (CK), C-reactive protein (CRP), direct bilirubin (DBIL), iron, gamma-glutamyl transferase (GGT), HDL cholesterol (HDLC), inorganic phosphorus (IP), LDL cholesterol (LDLC), total bilirubin (TBIL), total protein (TP), and uric acid (UA), as well as the performance of Sekisui assays for albumin, BUN, calcium, CRP, HDLC, IP, LDLC, TP, and UA by using Hitachi LABOSPECT 008 (Hitachi High-Tech Co., Japan).
METHODS
Liquid Assayed Multiqual Control (Bio-Rad Laboratories, USA) and pooled patients' sera were analyzed for 20 days. Wako linearity material (Wako Pure Chemical Industries, Ltd., Japan) and Sysmex Interference Check A Plus kit (Sysmex Co., Japan) were used to test linearity and interference, respectively. Concentrations of the target analytes were measured using Hitachi LABOSPECT 008 in 100 residual patient specimens and compared to those in Pureauto S series reagent (Sekisui Medical, Japan), which were measured using Hitachi 7600 (Hitachi High-Tech Co., Japan).
RESULTS
Total coefficients of variation (CVs) for the tested analytes were 0.91-9.26% in Wako and 1.04-7.46% in Sekisui assays. Linearity was demonstrated up to the highest concentration within the analytical range in all the assays except for Wako albumin and Sekisui TP. Wako and Sekisui albumin, BUN, CRP, HDLC, and LDLC assays, and in Wako C3, C4, calcium, and UA assays showed no interference with the test concentrations used. All the tested assays, except for Wako AST, LDLC, and TP, and Sekisui calcium and TP, demonstrated comparability with comparative method for at least one medical decision level.
CONCLUSIONS
Our study results showed that the analytical performances of Wako and Sekisui chemistry assays evaluated using Hitachi LABOSPECT 008 had appropriate analytical performance for clinical use.

Keyword

Chemistry Techniques, Analytical; Evaluation Studies; Clinical Chemistry Tests; Hitachi LABOSPECT 008

MeSH Terms

Alanine Transaminase
Aspartate Aminotransferases
Bilirubin
Blood Urea Nitrogen
C-Reactive Protein
Calcium
Chemical Industry
Chemistry Techniques, Analytical
Chemistry, Clinical
Cholesterol, HDL
Cholesterol, LDL
Clinical Chemistry Tests
Complement C3
Creatine Kinase
Humans
Iron
Phosphorus
Transferases
Uric Acid
Alanine Transaminase
Aspartate Aminotransferases
Bilirubin
C-Reactive Protein
Calcium
Cholesterol, HDL
Cholesterol, LDL
Complement C3
Creatine Kinase
Iron
Phosphorus
Transferases
Uric Acid

Figure

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  Fig. 1 Method comparison of Wako or Sekisui assays on Hitachi LABOSPECT 008 and Pureauto S assays on Hitachi 7600.

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  Fig. 2 Interference of common potential interfering substances in Wako and Sekisui assays on Hitachi LABOSPECT 008.

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