Lab Anim Res.  2013 Sep;29(3):178-181.

Inhibitory effects of a beta-dunnione compound MB12662 on gastric secretion and ulcers

• Jo IG
• Park D
• Kyung J
• Kim D
• Cai J
• Kim J
• Kwak TH
• Yoo SK
• Jeong HS
• Kim YB

Abstract

The effects of a beta-dunnione compound MB12662 on the gastric secretion and ulcers were investigated in rats. In order to assess the effects of MB12662 on the gastric secretion and acidity, rats were subjected to pylorus ligation operation, and 6 hours later, gastric fluid was collected. Treatment with MB12662 reduced the gastric fluid volume to 47.3% of control level and increased pH. In an alcohol-induced ulcer model, rats were orally administered 3 mL/kg of ethanol, and 1 hour later, the ulcer lesions ware measured under a stereomicroscope. MB12662 reduced ulcer index in a dose-dependent manner which was much stronger than a proton-pump inhibitor pantoprazole. In a stress-induced ulcer model, rats were subjected to water-immersion restraint stress, and 5 hours later, the ulcer lesions ware examined. MB12662 also attenuated the stress-induced gastric lesions, although the efficacy of MB12662 was lower than that of pantoprazole. Therefore, it is suggested that MB12662 could be a candidate compound for the prevention or treatment of gastric ulcers induced by gastric over-secretion and alcoholic hangover.

Keyword

Gastric secretion; ulcer; alcohol; stress; beta-dunnione; MB12662

MeSH Terms

2-Pyridinylmethylsulfinylbenzimidazoles
Alcoholics
Animals
Ethanol
Humans
Hydrogen-Ion Concentration
Ligation
Pylorus
Rats
Stomach Ulcer
Ulcer
2-Pyridinylmethylsulfinylbenzimidazoles
Ethanol

Figure

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  Figure 1 Representative findings of gastric ulcers induced by alcohol. Rats were orally administered with 3 mL/kg of pure ethanol, and sacrificed 1 hour later for the measurement of ulcer lesions. A, vehicle control; B, 10 mg/kg MB12662; C, 30 mg/kg MB12662; D, 10 mg/kg pantoprazole.

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  Figure 2 Effects of MB12662 (black) and pantoprazole (gray) on the gastric ulcer index (mm) induced by ethanol (3 mL/kg). *Significantly different from vehicle control, P<0.05.

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  Figure 3 Representative findings of gastric ulcers induced by water-immersion restraint stress (WIRS). Rats were placed in a restraint device, immersed up to their xiphoid process in a 22℃ water bath, and sacrificed 5 hours later for the measurement of ulcer lesions. A, vehicle control; B, 30 mg/kg MB12662; C, 10 mg/kg pantoprazole; D, 30 mg/kg pantoprazole.

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  Figure 4 Effects of MB12662 (black) and pantoprazole (gray) on the gastric ulcer index (mm) induced by water-immersion restraint stress (WIRS). *Significantly different from vehicle control, P<0.05.

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