Lab Anim Res.  2013 Mar;29(1):33-38.

A high resolution genetic mapping of the faded (fe) gene to a region between D10mit156 and D10mit193 on mouse chromosome 10

• Oh SH
• Nam H
• Suh JG

Abstract

The C57BL/6J-fe/fe mouse is a coat color mutant. The coat color of the homozygote mouse becomes progressively lighter with advancing age. The faded gene (fe) of C57BL/6J-fe/fe was mapped in a 2.0 cM distal to D10mit191 by our group. To make a high-resolution map, we used the Korean wild mouse (KWHM) for a backcross panel, which was captured in 1995 and has been maintained as an inbred line by our laboratory. In the inter-specific backcross panel (N=400), the fe gene was mapped to 1.0 cM distal to D10mit156. The gene order was defined: centromere -D10mit3/85 (1.3+/-0.6 cM)-D10mit155 (1.3+/-0.6 cM)-D10mit191 (2.0+/-0.7 cM)-D10mit156 (1.0+/-0.5 cM)-fe-D10mit193 (1.3+/-0.6 cM)-D10mit54 (1.0+/-0.5 cM)-D10mit44 (8.5+/-1.4 cM)-D10mit42 (10.0+/-1.5 cM). The measured distance between D10mit191 and D10mit 44 differed in both inter-specific (DBA/2) and intra-specific (KWHM) backcross panels (14.2 vs 13.8 cM). Taken together, our high-resolution linkage map of the fe locus from an intra-specific backcross panel will provide a good entry point to isolate the fe gene.

Keyword

Linkage analysis; hair color; faded mouse; haplotype analysis

MeSH Terms

Animals
Centromere
Chromosomes, Human, Pair 10
Gene Order
Hair Color
Homozygote
Mice

Figure

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  Figure 1 Age-related coat color change in C57BL/6J-fe/fe mice. The coat color of homozygous mice becomes progressively lighter with advancing age (black becoming gray).

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  Figure 2 The skin of the C57BL/6J (A) and C57BL/6J-fe/fe mice (B,C). In faded mice, the reduction of melanin content in hair bulbs with age is shown. H&E staining (× 400). M, Melanin; MC, Melanocyte; H, Hair.

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  Figure 3 Haplotype analysis of an interspecific backcross between fe and KWHM mice (C57BL/6J-fe/fe X KWHM)F1 X C57BL/6J-fe/fe. Each column represents a chromosomal haplotype identified in the backcross and inherited from the F1 parent. The number of individuals is listed beneath each column. Black boxes represent fe alleles, and white boxs represent KWHM alleles as determined by SSLP scoring with markers indicated at the left.

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  Figure 4 Genetic linkage maps of proximal chromosome 10 as derived from an interspecific backcross panel (C57BL/6J-fe/fe X KWHM)F1 X C57BL/6J-fe/fe) generated in this study. The distances of the intervals between loci and/or markers are not drawn to the same scale, and the locations of mapped human homologs are taken from the Mouse Genome Informatics (The Jackson Laboratory), Distances between intervals are shown in centimorgans±standard error to the left of the map. The linkage map of intra-specific backcross panel came from Oh et al., 2011[21].

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