Lab Anim Res.  2013 Mar;29(1):27-32.

Usefulness of a Helicobacter pylori stool antigen test for diagnosing H. pylori infected C57BL/6 mice

Affiliations
  • 1Center for Animal Resources Development, Wonkwang University, Iksan, Korea. kimoj@wku.ac.kr
  • 2Huvet. Inc., Iksan, Korea.
  • 3BioNote Inc., Hwaseong, Korea.
  • 4Department of Companion Animal and Animal Recourses Science, Joongbu University, Kummsan, Korea.

Abstract

Among several diagnostic tests, a Helicobacter pylori stool antigen (HpSA) test may offer a useful noninvasive method for diagnosing infection without sacrificing animals. In this study, male C57BL/6 mice (n=6) were infected with H. pylori ATCC 49503 (1x10(8) CFU/mouse) by intragastric inoculation three times at 2-day intervals, and H. pylori infected stool specimens were collected 1, 3, 5, 7, 14, 21 days after infection to assess reliability of the HpSA test. Five of six specimens were positive at 5-21 days after infection, and the sensitivity of the HpSA test was 83.33%. The presence of H. pylori infection was confirmed by the rapid urease test and genomic DNA polymerase chain reaction (PCR), and showed the same results as the HpSA. However, the rapid urease test and genomic DNA PCR are invasive tests and require animal sacrifice to detect H. pylori in gastric biopsy samples. We suggest that an HpSA test kit would be useful and effective for monitoring H. pylori in various laboratory animals, as H. pylori can be easily monitored without sacrificing animals.

Keyword

Helicobacter pylori; H. pylori stool antigen KIT; non-invasive; CLO; HpSA

MeSH Terms

Animals
Animals, Laboratory
Biopsy
Diagnostic Tests, Routine
DNA
Helicobacter
Helicobacter pylori
Humans
Male
Mice
Polymerase Chain Reaction
Urease
DNA
Urease

Figure

  • Figure 1 Results of Helicobacter pylori Stool Ag Kit based on the number of H. pylori ATCC 49503 cells. (A) 1×105 CFU/100 µL; (B) 1×106 CFU/100 µL; (C) 1×107 CFU/100 µL; (D) 1×108 CFU/100 µL.


Reference

1. Suerbaum S, Michetti P. Helicobacter pylori infection. N Engl J Med. 2002; 347(15):1175–1186. PMID: 12374879.
2. Dunn BE, Cohen H, Blaser MJ. Helicobacter pylori. Clin microbiol Rev. 1997; 10(4):720–741. PMID: 9336670.
Article
3. Warren JR, Marshall B. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983; 1(8336):1273–1275. PMID: 6134060.
4. Basset C, Holton J, Vaira D. Helicobacter: a paradigm shift in peptic ulcer disease and more? Sci Prog. 2002; 85(Pt 1):13–31. PMID: 11969117.
Article
5. Linz B, Balloux F, Moodley Y, Manica A, Liu H, Roumagnac P, Falush D, Stamer C, Prugnolle F, van der, Yamaoka Y, Graham DY, Perez-Trallero E, Wadstrom T, Suerbaum S, Achtman M. An African origin for the intimate association between humans and Helicobacter pylori. Nature. 2007; 445(7130):915–918. PMID: 17287725.
Article
6. Park DW, Lee KJ, Jin SH, Lee JH, Min JS, Park SH, Yu HJ, Bang HY, Lee JI. Phenotypic Differences of Gastric Cancer according to the Helicobacter pylori Infection in Korean Patients. J Gastric Cancer. 2010; 10(4):168–174. PMID: 22076182.
7. Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M, Taniyama K, Sasaki N, Schlemper RJ. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001; 345(11):784–789. PMID: 11556297.
8. Polk DB, Peek RM Jr. Helicobacter pylori: gastric cancer and beyond. Nat Rev Cancer. 2010; 10(6):403–414. PMID: 20495574.
Article
9. International Agency for Research on Cancer (IARC). Schistosomes, liver flukes and Helicobacter pylori. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. 1994. Geneva, Switzerland: World Health Organization;p. 177–220.
10. Park DW, Lee KJ, Jin SH, Lee JH, Min JS, Park SH, Yu HJ, Bang HY, Lee JI. Phenotypic Differences of Gastric Cancer according to the Helicobacter pylori Infection in Korean Patients. J Gastric Cancer. 2010; 10(4):168–174. PMID: 22076182.
11. Anwar MM, Youssef AI, Sheta MI, Zaki A, Bernaba NR, El-Toukhi MA. Evaluation of specific biochemical indicators of Helicobacter pylori-associated gastric cancer in Egypt. East Mediterr Health J. 2012; 18(5):501–507. PMID: 22764438.
12. Hu LT, Mobley HL. Purification and N-terminal analysis of urease from Helicobacter pylori. Infect Immun. 1990; 58(4):992–998. PMID: 2318539.
Article
13. Eaton KA, Brooks CL, Morgan DR, Krakowka S. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect Immun. 1991; 59(7):2470–2475. PMID: 2050411.
Article
14. Mégraud F. The most important diagnostic modalities for Helicobacter pylori, now and in the future. Eur J Gastroenterol Hepatol. 2012; 9(Suppl 1):S13–S15. PMID: 22498901.
Article
15. Leal YA, Flores LL, Fuentes-Pananá EM, Cedillo-Rivera R, Torres J. 13C-urea breath test for the diagnosis of Helicobacter pylori infection in children: a systematic review and metaanalysis. Helicobacter. 2011; 16(4):327–337. PMID: 21762274.
Article
16. Nyan DC, Welch AR, Dubois A, Coleman WG Jr. Development of a noninvasive method for detecting and monitoring the time course of Helicobacter pylori infection. Infect Immun. 2004; 72(9):5358–5364. PMID: 15322033.
17. Falsafi T, Favaedi R, Mahjoub F, Najafi M. Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children. World J Gastroenterol. 2009; 15(4):484–488. PMID: 19152455.
Article
18. Santos AM, Lopes T, Oleastro M, Chaves P, Cordeiro R, Ferreira M, Pereira T, Machado J, Guerreiro AS. Role of 13C-urea breath test in experimental model of Helicobacter pylori infection in mice. Helicobacter. 2011; 16(4):320–326. PMID: 21762273.
Article
19. Hirayama F, Takagi S, Yokoyama Y, Iwao E, Ikeda Y. Establishment of gastric Helicobacter pylori infection in Mongolian gerbils. J Gastroenterol. 1996; 31(Suppl 9):24–28. PMID: 8959513.
20. Zhang S, Moss SF. Rodent models of Helicobacter infection, inflammation, and disease. Methods Mol Biol. 2012; 921:89–98. PMID: 23015495.
Article
21. Ghiara P, Rossi M, Marchetti M, Di Tommaso A, Vindigni C, Ciampolini F, Covacci A, Telford JL, De Magistris MT, Pizza M, Rappuoli R, Del Giudice G. Therapeutic intragastric vaccination against Helicobacter pylori in mice eradicates an otherwise chronic infection and confers protection against reinfection. Infect Immun. 1997; 65(12):4996–5002. PMID: 9393788.
Article
22. Crabtree JE. Eradication of chronic Helicobacter pylori infection by therapeutic vaccination. Gut. 1998; 43(1):7–8. PMID: 9771398.
Article
23. Gómez-Duarte OG, Bumann D, Meyer TF. The attenuated Salmonella vaccine approach for the control of Helicobacter pylori-related diseases. Vaccine. 1999; 17(13-14):1667–1673. PMID: 10194821.
Article
24. Ruiz-Bustos E, Sierra-Beltran A, Romero MJ, Rodriguez-Jaramillo C, Ascencio F. Protection of BALB/c mice against experimental Helicobacter pylori infection by oral immunisation with H pylori heparan sulphate-binding proteins coupled to cholera toxin beta-subunit. J Med Microbiol. 2000; 49(6):535–541. PMID: 10847207.
25. Wang X, Hirmo S, Willén R, Wadström T. Inhibition of Helicobacter pylori infection by bovine milk glycoconjugates in a BAlb/cA mouse model. J Med Microbiol. 2001; 50(5):430–435. PMID: 11339250.
Article
26. Wada T, Aiba Y, Shimizu K, Takagi A, Miwa T, Koga Y. The therapeutic effect of bovine lactoferrin in the host infected with Helicobacter pylori. Scand J Gastroenterol. 1999; 34(3):238–243. PMID: 10232866.
27. Lee HA, Hong S, Oh HG, Park SH, Kim YC, Park H, Jeong GS, Kim O. Antibacterial Activity of Sanguisorba officinalis against Helicobacter pylori. Lab Anim Res. 2010; 26(3):257–263.
28. Varbanova M, Malfertheiner P. Bacterial load and degree of gastric mucosal inflammation in Helicobacter pylori infection. Dig Dis. 2011; 29(6):592–599. PMID: 22179216.
29. Graham DY, Fischbach L. Helicobacter pylori treatment in the era of increasing antibiotic resistance. Gut. 2010; 59(8):1143–1153. PMID: 20525969.
Article
30. Gisbert JP, Pajares JM. Treatment of Helicobacter pylori infection: the past and the future. Eur J Intern Med. 2010; 21(5):357–359. PMID: 20816583.
Article
31. Vaira D, Holton J, Menegatti M, Ricci C, Landi F, Ali' A, Gatta L, Acciardi C, Farinelli S, Crosatti M, Berardi S, Miglioli M. New immunological assays for the diagnosis of Helicobacter pylori infection. Gut. 1999; 45(Suppl 1):I23–I27. PMID: 10457032.
Article
32. Hoshina S, Kahn SM, Jiang W, Green PH, Neu HC, Chin N, Morotomi M, LoGerfo P, Weinstein IB. Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies. Diagn Microbiol Infect Dis. 1990; 13(6):473–479. PMID: 1703940.
Article
33. Kazemi S, Tavakkoli H, Habizadeh MR, Emami MH. Diagnosticvalues of Helicobacter pylori diagnostic tests: stool antigen test, urea breath test, rapid urease test, serology and histology. J Res Med Sci. 2011; 16(9):1097–1104. PMID: 22973378.
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