Lab Anim Res.  2010 Jun;26(2):133-137.

Determining NOEL/NOAEL in Repeated-dose Toxicity Studies, When the Low Dose Group Shows Significant Difference in Quantitative Data

Affiliations
  • 1National Institute of Technology and Evaluation (NITE), Tokyo, Japan. kobayashi-katsumi@nite.go.jp
  • 2Frontier Lifeline, International Centre for Cardio-Thoracic and Vascular Diseases, Chennai, India.

Abstract

In repeated-dose 28-day oral toxicity study design, the low dose is fixed as the no observed effect level (NOEL). But, in practice the low dose usually shows significant difference in few measurable items in most of the studies. We investigated 109 of repeated-dose 28-day oral toxicity studies in rats conducted according to the Chemical Substance Control Law, Japan and examined the measurable items (functional observational battery, urinalysis, hematology, blood chemistry and absolute and relative organ weights) of the low dose group which showed a statistical significant difference (P<0.05) compared to the respective control groups. The investigation revealed that, 205/12,167 (1.6%) measurable items showed a significant difference in the low dose groups. The significant difference shown by urinalysis was high (3.3%), followed by clinical chemistry parameters, hematology, relative organ weights and absolute organ weights (1.8-1.1%). We conclude from the investigation that the low dose may be considered as NOEL, if the significant difference of measurable items of it is about 2% (maximum <5%), compared to the control. However, due consideration may be given to the clinical relevance of the items that showed a significant difference.

Keyword

Repeated-dose toxicity study; statistical significant difference; rats; low dose; NOEL/NOAEL

MeSH Terms

Animals
Chemistry, Clinical
Hematology
Japan
Jurisprudence
No-Observed-Adverse-Effect Level
Organ Size
Rats
Urinalysis

Reference

Kobayashi K.., Pillai K.S.., Sakuratani Y.., Abe T.., Kamata E.., Hayashi M.2008. Evaluation of statistical tools used in short-term repeated dose administration toxicity studies with rodents. J. Toxicol. Sci. 33(1):97–104.
Article
Kobayashi K.., Sakuratani Y.., Abe T.., Nishikawa S.., Yamada J.., Hirose A.., Kamata E.., Hayashi M.2010. Relation between statistics and treatment-related changes obtained from toxicity studies in rats: if detected a significant difference in low or middle dose for quantitative values, this change is considered as incidental change? J. Toxicol. Sci. 35(1):79–85.
Article
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NITE. 2007. http://www.safe.nite.go.jp/kasinn/pdf/28test.pdf.
OECD. 1995. Organization for Economic Cooperation and Development. OECD Guidelines for Testing of Chemicals. Repeated Dose 28-Day Oral Toxicity Study in Rodents. No. 407, p. 8, OECD, Paris.
OECD. 2000. Organization for Economic Cooperation and Development. Guidance Notes for Analysis and Evaluation of Repeat-Dose Toxicity Studies. OECD Environment, Health and Safety Publications Series on Pesticides No. 10. ENV/JM/MONO/2000 (18), p.87. OECD, Paris.
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