Lab Med Online.  2016 Jul;6(3):176-182. 10.3343/lmo.2016.6.3.176.

Comprehensive Analysis of Blood Cultures Using BacT/Alert 3D FA Plus and FN Plus Media

Affiliations
  • 1Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, Korea. sjkim8239@hanmail.net
  • 2Department of Convergence of Medical Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
  • 3Department of Emergency Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.

Abstract

BACKGROUND
We prospectively evaluated the performance of blood culture resin media, FA Plus and FN Plus, of the BacT/Alert 3D System (bioMérieux Inc., USA) in a tertiary university-affiliated hospital.
METHODS
We obtained 2,994 blood culture sets. The positivity and time to detection (TTD) were compared between FA Plus and FN Plus for clinically significant microorganisms. We then categorized patients into two groups based on antibiotic treatment before blood culture to observe the difference of positivity between two groups.
RESULTS
Among 2,994 sets received, 371 (12.4%) yielded 385 clinically significant pathogens. Comparing FA Plus to FN Plus media, lactose non-fermenters (18 vs. 1; P<0.001) and yeasts (15 vs. 0; P<0.001) were recovered at a significantly higher rate using FA Plus, whereas gram-positive cocci (10 vs. 26; P=0.011) and gram-negative anaerobes (0 vs. 10 P=0.002) were isolated at a higher rate using FN Plus. In terms of detection time in FA Plus compared to that in FN Plus, Staphylococcus aureus (13.8 hr vs. 18.1 hr) and gram-positive cocci (14.1 hr vs. 20.3 hr) were detected significantly earlier in FA Plus (each, P<0.001). There was no statistically significant difference in positivity based on prior antibiotic use (13.1%, 65/498) compared to antibiotic naïve patients (12.3%, 306/2,496) (P>0.05).
CONCLUSIONS
Complementary detection of microorganisms was observed between FA Plus and FN Plus. Gram-positive cocci including S. aureus grew faster in FA Plus. In addition, the rate of positivity was not affected by prior antibiotic therapy in BacT/Alert 3D resin media.

Keyword

Sepsis; Blood culture; Resin; Time to detection

MeSH Terms

Gram-Positive Cocci
Humans
Lactose
Prospective Studies
Sepsis
Staphylococcus aureus
Yeasts
Lactose

Figure

  • Fig. 1 Cumulative curves of time to detection (TTD) in blood culture bottles. FA Plus and FN Plus of the BacT/Alert 3D System.


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