Korean J Blood Transfus.  2006 Jun;17(1):71-76.

Detection of an AB(weak) Blood Group by Genotyping

Affiliations
  • 1Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea. kshanmd@snu.ac.kr
  • 2Department of Laboratory Medicine, Sung Ae Hospital1, Seoul, Korea.

Abstract

Since precise ABO blood group typing is the most important part of a safe blood transfusion, the cause of an ABO discrepancy should be determined before issuing blood. The authors confirmed the ABO blood group of a 25-year-healthy male using genotyping method in those who showed an ABO blood group discrepancy between the cell and serum types. ABO typing, saliva test, adsorption elution test and serum transferase assay were performed and the presence of a weak B substance and B transferase was suspected without any evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) analysis was performed using the restriction enzymes including BstE II, Pvu II, BssH II, Alu I, and Mva I. The genotyping result showed a normal A allele and B allele. DNA analysis of exons 6 and 7 revealed normal A and B sequences. Therefore, we confirmed A(1)B(weak) blood type.

Keyword

ABO blood group; Genotyping; PCR-RFLP

MeSH Terms

Adsorption
Alleles
Blood Transfusion
DNA
Erythrocytes
Exons
Humans
Male
Saliva
Transferases
DNA
Transferases
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