Korean J Blood Transfus.  2006 Jun;17(1):39-47.

Comparison of Amicus and CS-3000 Plus for the Collection of Peripheral Blood Stem Cells

Affiliations
  • 1Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dwryang@chonnam.ac.kr
  • 2Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
  • 3Department of Occupational and Environmental Medicine, Chonnam National University Medical School, Gwangju, Korea.

Abstract

BACKGROUND: The efficient collection of peripheral blood stem cells (PBSC) from donors who donate for allogeneic transplants as well as from patients undergoing autologous transplants is essential for a successful transplant. Recently, the Amicus cell separator and the associated MNC collection computer software program for PBSC collection were introduced in Korea.
METHODS
Two apheresis machines (Amicus, Baxter Healthcare; and CS-3000 plus, Baxter Healthcare) were compared retrospectively. A total number of 144 procedures were performed on 14 donors and 28 patients. The pre- and post-apheresis complete blood cell (CBC) counts and the number of hematopoietic progenitor cells (HPC) were determined in the peripheral blood from the subjects. The CBC, HPC, CD34+ cell counts and the level of colony-forming unit-granulocyte-macrophages (CFU-GM) were measured in the PBSC product collected from both machines.
RESULTS
Both machines collected a similar number of CD34+ cells from the donors and patients. On the other hand, the Amicus collected significantly more nucleated cells, MNCs, HPCs and CFU-GM in the patients with significantly less RBC contamination than those with CS-3000 plus. The decrease in the peripheral blood platelet counts in the donors and patients was more prominent after apheresis using the CS-3000 plus (117.00+/-42.75 x 10(3)/microliter, 61.22+/-43.62 x 10(3)/microliter) than Amicus (26.04+/-18.68 x 10(3)/microliter, 22.15+/-28.66 x 10(3)/microliter)(p<0.05).
CONCLUSION
PBSC collection can be performed successfully using CS-3000 plus and Amicus. Amicus is superior to CS-3000 plus in avoiding apheresis-induced thrombocytopenia, and is expected to prevent unnecessary platelet transfusion.

Keyword

Peripheral blood stem cell collection; Amicus; CS-3000 plus; Platelet contamination

MeSH Terms

Autografts
Blood Cells
Blood Component Removal
Cell Count
Delivery of Health Care
Granulocyte-Macrophage Progenitor Cells
Hand
Hematopoietic Stem Cells
Humans
Korea
Platelet Count
Platelet Transfusion
Retrospective Studies
Stem Cells*
Thrombocytopenia
Tissue Donors
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