Korean J Blood Transfus.  2009 Dec;20(3):227-234.

Comparison of ABO Isoagglutinin Titers by Different Tube Hemagglutination Techniques

• Kang MG
• Lee SJ
• Oh JS
• Lim YA

Abstract

BACKGROUND
The ABO isoagglutinin titer is useful for the evaluation and observation of ABO incompatible bone marrow transplantation or organ transplantation, yet the results can be different depending on the test methods. Measurement of isoagglutinin titer using the gel test has recently been reported, but this test is expensive. In this study, we investigated isoagglutinin titer distribution of normal individuals using the different tube hemagglutination technique to help select the best test method and to interpret the agglutinin titer.
METHODS
Normal healthy individuals were selected from those patients who underwent a physical examination at Ajou University Hospital, during July 2009. Sixty healthy individuals, (10 men and 10 women per each ABO blood group) were recruited for the study. The immediate spin method (IS), the anti-human globulin method with dithiothreitol treatment (DTT-AHG) and the anti-human globulin method without DTT treatment (AHG) were performed simultaneously. The reciprocal of the highest serum dilution that showed macroscopic agglutination 1+ or more was regarded as the isoagglutinin titer.
RESULTS
The isoagglutinin titer measured by the AHG was the highest in the all blood groups. In case of blood groups A and B, the isoagglutinin titer by the IS was higher than that by the DTT-AHG, but this was quite the reverse in the case of the blood group O.
CONCLUSION
If it is not necessary to distinguish IgM antibody and IgG antibody, then it seems that the AHG is the best practical method of those three methods. It was more sensitive than the IS and more rapid and easier than the DTT-AHG.

Keyword

ABO isoagglutinin titer; Tube hemagglutination technique; IgG

MeSH Terms

Agglutination
Blood Group Antigens
Bone Marrow Transplantation
Dithiothreitol
Female
Hemagglutination
Humans
Immunoglobulin G
Immunoglobulin M
Male
Organ Transplantation
Physical Examination
Transplants
Blood Group Antigens
Dithiothreitol
Immunoglobulin G
Immunoglobulin M