Korean J Blood Transfus.  2010 Apr;21(1):74-79.

Auto-antibody Showing Anti-Fy(b) Specificity as Proven by the Dilution Method in the Presence of Warm Autoantibodies: A Case Report

• Jeong ES
• Jang MJ
• Cho D
• Lim CS
• Ryang DW

Abstract

Several approaches have been introduced to detect allo-antibodies in the presence of warm auto-antibodies, and these methods include warm autoadsorption, cysteine-activated papain and dithiothreitol (ZZAP), and polyethylene glycol (PEG) and dilution of the patient's serum. Among them, the dilution technique is a simple and rapid method. During pretransfusion testing of a 33 year-old systemic lupus erythematosus (SLE) patient with warm auto-antibodies, antibody identification was done by the dilution technique with using serum diluted 1-in-8. The patient demonstrated an anti-Fy(b) pattern of reactivity in his sera. Contrary to our expectations, the phenotype of the erythrocytes was Fy(a+/b+) and the genotype, as assessed by performing PCR-restriction fragment length polymorphism (RFLP), was FY*A/FY*B. These results suggest that the antibody is an autoantibody showing anti-Fy(b) specificities. An antibody identification test using undiluted serum showed the same result when 40 days had passed. We report here on a case with auto-anti-Fy(b) proven by the dilution method in the presence of warm autoantibodies.

Keyword

Anti-Fy(b); Dilution method; Warm autoantibody

MeSH Terms

Autoantibodies
Dithiothreitol
Erythrocytes
Genotype
Humans
Indicator Dilution Techniques
Lupus Erythematosus, Systemic
Papain
Phenotype
Polyethylene Glycols
Sensitivity and Specificity*
Autoantibodies
Dithiothreitol
Papain
Polyethylene Glycols