Increased PMA-stimulated Release of MMP-9 and TGF-beta1 in the Plasma of Children with Persistent Asthma

Abstract

BACKGROUND
The contribution of matrix metalloproteinase (MMP)-9 to the pathogenesis of asthma has been reported. Transforming growth factor (TGF)-beta1 and interleukin (IL)- 11, and -17 are profibrotic cytokines which increase in severe asthma.
OBJECTIVE
The aim of our study was to determine whether the release of these enzyme and cytokines in young children with persistent asthma during acute exacerbation and investigate if there is any difference compared with intermittent asthma. METHOD: Young children less than 6 years who were admitted with acute exacerbation of asthma were enrolled. Two groups of asthmatic patients were defined; the patients who had had persistent symptoms since the onset of disease (Group 1) and the patients who had had intermittent asthma symptoms (Group 2). Phorbol myristate acetate (PMA)-stimulated MMP-9, TGF-beta1, IL-11 and IL-17 were measured by ELISA in the plasma obtained on admission in both patient groups and controls. RESULT: Both MMP-9 and TGF-beta1 increased significantly in the patients with persistent asthma of group 1 compared with group 2 and controls. A significant correlation was found between MMP-9 and TGF-beta1 in group 1. No significant difference was found in IL-11 and IL-17 levels between asthmatic patients and controls. CONCLUSION: Our result demonstrated that persistent asthma is associated with increased PMA-induced plasma MMP-9 and TGF-beta1 compared with intermittent asthma during acute exacerbation, and suggests that the mechanism of acute airway inflammation may be quite different between these two pediatric asthma groups.

Increased PMA-stimulated Release of MMP-9 and TGF-beta1 in the Plasma of Children with Persistent Asthma

Abstract

MeSH Terms

Cited

Save citations to file

Email citations