Korean J Asthma Allergy Clin Immunol.  2009 Sep;29(3):194-199.

Intranasal Administration of Unmethylated CpG with Cockroach Antigen Prevents the Development of Cockroach-Induced Allergic Inflammation

Abstract

BACKGROUND
Unmethylated CpG can induce Th1 and regulatory T cell immune responses and may modulate ovalbumin-induced mouse allergic asthma. However, the effects of CpG in a cockroach allergen asthma model have not yet been elucidated.
OBJECTIVE
To evaluate whether unmethylated CpG can prevent the development of indoor allergen-induced allergic asthma.
METHODS
Effects of CpG were evaluated using a cockroach allergen-induced mouse model. CpG and Blattella germanica (CR) allergen were co-administered intranasally, and methacholine airway hyperresponsiveness (MCh-AHR), inflammatory cells and cytokine profiles in BAL fluid and lung histology were evaluated. RESULT: Co-administration of CpG with CR allergen can prevent the development of MCh-AHR and allergic inflammation in a CR-induced asthma mouse model. Eosinophils, lymphocytes, macrophages and neutrophils in BAL fluid were decreased by the co-administration of unmethylated CpG in an OVA-induced asthma model. The expression of IL-5, IL-13 and IFN-gamma in BAL fluid was attenuated by the CpG. Peribronchial, perivascular inflammation, and goblet cell hyperplasia in respiratory epithelium were also markedly attenuated by the CpG.
CONCLUSION
These findings suggest that unmethylated CpG may have role in the immune modulation of allergic asthma induced by indoor allergens.


MeSH Terms

Administration, Intranasal
Animals
Asthma
Cockroaches
Eosinophils
Goblet Cells
Hyperplasia
Inflammation
Interleukin-13
Interleukin-5
Lung
Lymphocytes
Macrophages
Methacholine Chloride
Mice
Neutrophils
Respiratory Mucosa
Interleukin-13
Interleukin-5
Methacholine Chloride
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