Korean Circ J.  2011 Oct;41(10):590-595. 10.4070/kcj.2011.41.10.590.

Angiopoietin-1 Gene Therapy Attenuates Hypertension and Target Organ Damage in Nitric Oxide Synthase Inhibited Spontaneously Hypertensive Rats

Affiliations
  • 1College of Pharmacy, Ajou University, Suwon, Korea.
  • 2Department of Medicine, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, Korea. dkkim@skku.edu
  • 3Biomedical Research Center and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.

Abstract

BACKGROUND AND OBJECTIVES
In our previous study, we found that the gene transfer of a potent derivative of cartilage oligomeric matrix protein Angiopoietin-1 (COMP-Ang-1) substantially prevented hypertension, microvascular rarefaction, and target organ damage in spontaneously hypertensive rats (SHRs). The purpose of the present study was to examine the role of nitric oxide (NO) in the therapeutic effects observed after COMP-Ang-1 gene transfer.
MATERIALS AND METHODS
To exclude the NO-mediated effects in COMP-Ang-1 gene therapy, the SHRs were treated with an NO synthase (NOS) inhibitor, Nw-nitro-L-arginine methyl ester (L-NAME) before the electrophoretic gene transfer.
RESULTS
The pretreatment with L-NAME induced a severe and sustained increase in systolic blood pressure (BP) in a LacZ plasmid transferred control SHR. However, the electrophoretic transfer of a COMP-Ang-1 plasmid instead of LacZ plasmid in L-NAME-pretreated SHRs substantially blocked the development of hypertension without any significant difference in comparison with L-NAME-untreated COMP-Ang-1 plasmid transferred groups. In addition, the COMP-Ang-1 plasmid transfer substantially attenuated microvascular rarefaction and arteriole remodeling in the heart and kidney, which might account for the mild histological alterations observed in the COMP-Ang-1 plasmid transferred group, in contrast to the severe fibrosis and necrosis seen in the LacZ plasmid controls.
CONCLUSION
These therapeutic outcomes of COMP-Ang-1 gene transfer even in NOS inhibited SHRs suggested that the antihypertensive effect of COMP-Ang-1 was not merely secondary to NO-mediated vasorelaxation, but it may be associated with its ability to protect the vascular endothelium probably via an NO-independent mechanism which serves to attenuate microvascular rarefaction and target organ damage, and also to prevent hypertension by reducing peripheral vascular resistance.

Keyword

Angiopoietin-1; Endothelium; Hypertension; Nitric oxide

MeSH Terms

Angiopoietin-1
Arterioles
Blood Pressure
Cartilage
Endothelium
Endothelium, Vascular
Extracellular Matrix Proteins
Fibrosis
Genetic Therapy
Glycoproteins
Heart
Hypertension
Kidney
Necrosis
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Plasmids
Rats, Inbred SHR
Vascular Resistance
Vasodilation
Angiopoietin-1
Extracellular Matrix Proteins
Glycoproteins
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase

Figure

  • Fig. 1 pCOMP-Ang-1 gene transfer ameliorates hypertension in NOS inhibited SHRs. A: a significant hypotensive effect of pCOMP-Ang-1 gene transfer was seen in L-NAME-pretreated SHRs. No significant difference (p=0.0876, n=11) was observed between L-NAME-pretreated pCOMP-Ang-1-transferred SHRs (pCOMP-Ang-1+L-NAME, 161.8±2.4 mm Hg) and L-NAME untreated pCOMP-Ang-1-transferred SHRs (pCOMP-Ang-1, 154.2±2.9 mm Hg). However, systolic BP of pCOMP-Ang-1+L-NAME SHRs was significantly (n=11) lower than that of L-NAME pretreated pLacZ-transferred SHRs (pLacZ+L-NAME, 162.3±3.7 mm Hg). The systolic BP of SHRs (in mm Hg) was measured using the tail-cuff method just before (white bar) and three weeks after (black bar) gene transfer. B: no significant difference (NS) was seen in the plasma concentration of nitrites between pLacZ+L-NAME (12.2±0.6 mM) and pCOMP-Ang-1+L-NAME SHRs (12.1±1.0 mM) (n=11).

  • Fig. 2 pCOMP-Ang-1 gene transfer reduces microvascular rarefaction in NOS inhibited SHRs. The microvasculature of the heart (A: red arrowheads indicating the absence of blood vessels) and kidney (C: red arrowheads indicating the loss of glomerular capillaries) was visualized by immunohistochemical staining with CD31 antibody for frozen sections or RECA antibody for paraffin sections. B: the capillary density in the heart section was quantified per myofiber (1.3±0.04 cells/myofiber in pCOMP-Ang-1+L-NAME, 0.8±0.09 cells/myofiber in pLacZ+L-NAME, *p<0.05, n=7). Scale bar is 10 µm.

  • Fig. 3 pCOMP-Ang-1 gene transfer attenuates myocardial and renal damage in NOS inhibited SHRs. Representative photomicrographs of the MT-stained heart (A: black arrowheads indicating the multifocal myocardial fibrosis) and kidney (C: black arrowheads indicating the tubular interstitial fibrosis) with high-power images of small arterioles (right bottom insets). B: the fibrotic area shown in the MT-stained heart sections was quantified as a percentage relative to the total tissue area (20.7±3.0% in pCOMP-Ang-1+L-NAME, 42.2±0.08% in pLacZ+L-NAME, *p<0.05, n=7). D: Kidney function was determined by measuring the amount of protein in the urine collected at the end of the experiment (1.0±0.1 mg/mL in pCOMP-Ang-1+L-NAME, 1.6±0.03 mg/mL in pLacZ+L-NAME, *p<0.05, n=4). Scale bar is 10 µm.


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