Korean J Urol.  2005 Dec;46(12):1344-1347.

Expression of the mRNA of Growth Factor Receptors and Neurotrophic Factors in Porcine Vesical Submucosa and Acellular Matrix

Affiliations
  • 1Department of Urology, Sungkyunkwan University School of Medicine, Seoul, Korea. hjae.park@samsung.com

Abstract

PURPOSE: Several naturally-derived biopolymers have been introduced as implantable biomaterial for tissue reconstruction, and some of them are already being applied in various clinical fields. They serve as backbones for the regeneration of damaged tissue. It is well known that growth factors play an important role in this process and some of the naturally- derived biopolymers were known to contain several kinds of growth factors, but there are few reports about any growth factors in the various biopolymers. We tried to confirm the existence of growth factor receptors and neurogenic factors through the expression of the mRNA from the biopolymers.
MATERIALS AND METHODS
Bladders were harvested from adult pigs. Vesical submucosa was obtained with using mechanical decellulization technique. Acellular matrices were made from pig bladder with using a cell lysis technique, and they were examined by performing scanning electron microscopy. Expression of the mRNA of vascular endothelial growth factor (VEGF) receptor, fibroblast growth factor (FGF)-1 receptor, neuregulin and brain-derived neurotrophic factor (BDNF) in the acellular matrix and the submucosa of porcine bladder were examined for via reverse transcription- polymerase chain reaction (RT-PCR).
RESULTS
The ultrastructure of the acelluar matrix shows collagen fibers with many pores. The mRNA's of VEGF receptor, FGF-1 receptor and neuregulin were expressed in the porcine vesical acellular matrix, whereas BDNF was not expressed. On the other hand, none of the mRNA's examined was expressed in the porcine vesical submucosa.
CONCLUSIONS
This study's results shows that several growth factors are detected in the acellular matrix of the porcine bladder. These growth factors may play an important role for the growth of tissue when it is implanted in vivo. It is also assumed that there are more growth factors in naturally-derived biopolymers, and it would be useful to investigate the undefined components of naturally-derived biopolymers for developing new and advanced polymers.

Keyword

Matrix; Bladder; Biopolymers

MeSH Terms

Adult
Biopolymers
Brain-Derived Neurotrophic Factor
Collagen
Fibroblast Growth Factor 1
Fibroblast Growth Factors
Hand
Humans
Intercellular Signaling Peptides and Proteins
Microscopy, Electron, Scanning
Nerve Growth Factors*
Polymerase Chain Reaction
Polymers
Receptors, Growth Factor*
Receptors, Vascular Endothelial Growth Factor
Regeneration
RNA, Messenger*
Swine
Urinary Bladder
Vascular Endothelial Growth Factor A
Biopolymers
Brain-Derived Neurotrophic Factor
Collagen
Fibroblast Growth Factor 1
Fibroblast Growth Factors
Intercellular Signaling Peptides and Proteins
Nerve Growth Factors
Polymers
RNA, Messenger
Receptors, Growth Factor
Receptors, Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor A
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