Korean J Urol.
2002 Dec;43(12):1065-1069.
Expression of Heat Shock Protein 70 in Ipsilateral Rat Kidney with Unilateral Partial Ureteral Obstruction
- Affiliations
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- 1Department of Urology, Yonsei University Wonju College of Medicine, Wonju, Korea.
- 2Department of Urology, College of Medicine, Konyang University, Daejeon, Korea.
Abstract
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PURPOSE: A partial ureteral obstruction is a common clinical finding. Heat shock proteins (HSPs) maintain homeostasis, and protect cells from various injuries caused by physical and biochemical stresses. Particularly, heat shock protein 70 (HSP70) is contained within renal tissue, and is attributed to minimizing injuries to renal tissue. We tried to understand the effect of renal cellular injuries due to a unilateral partial ureteral obstruction (UPUO). This was done by determining the expression of HSP70, using immunohistochemical staining, to identify intracellular changes in the kidney of rats with UPUO.
MATERIALS AND METHODS
The left kidney of male Sprague-Dawley rats (250-300gm) were used, the UPUO group was assigned a UPUO operation (n=20), and the control group was assigned to a sham operation (n=20). A left nephrectomy was performed in both groups 7 days after the operations. The intensity of the HSP70 in the tissue stained by immunohistochemical staining was measured using an image analyzer (Multiscan, USA).
RESULTS
In the control group, the expression of the HSP70 was weak in the glomerular area. However, in the UPUO group, the expression was stronger than that of the control group in the glomerular area. The mean intensity in the UPUO group (182.1+/-11.9) was significantly higher than in the controls (189.6+/-7.9) (p<0.05).
CONCLUSIONS
The stronger expression of HSP70 in the UPUO group suggests that HSP70 may be a useful indicator for the cellular damage caused by a partial ureteral obstruction.