Korean J Reprod Med.  2010 Mar;37(1):13-23.

Differentiation of Human Embryonic Stem Cells into Germ Cell and Culture Condition for Single Embryonic Stem Cells Dissociated by Enzyme

Affiliations
  • 1Mizmedi Hospital ART Research Center, Korea. ivf129@mizmedi.net

Abstract


OBJECTIVE
The present study was carried out to induce differentiation of human embryonic stem cells (hESCs) into germ cells and to establish a culture condition for single hESCs dissociated by enzyme.
METHODS
Embryonic body (EB) was formed by hanging drop culture for 3 days from hESCs colony. The EBs were cultured in the medium supplemented with retionic acid (RA) or/and bone morphogenetic protein-4 (BMP4) for 14 days to differentiate into germ cells. Germ cell specific markers, c-kit and VASA were used for immunohistochemistry of EB. Human ESCs colonies were dissociated into single cells by Collagenase, Tryple and Accutase, and then colony formation rate of the single cells was examined. Rho-associated kinase inhibitor (ROCK inhibitor, Y27632) was added into the culture medium of single cells to reduce the apoptotic damage during the dissociation.
RESULTS
Single cells dissociated with Tryple or Accutase showed higher colony formation rates compared to the cells dissociated with Collagenase. Seeding of 5x10(3) cells/well (4 well dish) was efficient to obtain high colony formation rate compared to other concentrations of seeding cell. Addition of Y27632 significantly increased the colony formation rate of the single cells dissociated by Tryple. Immunohistochemistry of EB with c-kit and VASA markers showed a weak fluorescence signals compared to the signals from the testicular tissue.
CONCLUSION
Dissociation with Tryple was useful to obtain healthy single cells and addition of Y27632 was beneficial for survival and colony formation of the single cells. Unlike other studies, we just observed a dim fluorescence staining of the germ cell markers, probably caused by the short-term culture for the differentiation of EB compared to other studies.

Keyword

HESCs; Germ cells; Tryple; Retionic acid; Y27632

MeSH Terms

Amides
Collagenases
Dissociative Disorders
Embryonic Stem Cells
Fluorescence
Germ Cells
Humans
Immunohistochemistry
Peptide Hydrolases
Pyridines
rho-Associated Kinases
Seeds
Amides
Collagenases
Peptide Hydrolases
Pyridines
rho-Associated Kinases
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